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微型观赏石榴组培快繁技术
引用本文:王云波,郑扑红. 微型观赏石榴组培快繁技术[J]. 北方园艺, 2011, 0(3): 153-155
作者姓名:王云波  郑扑红
作者单位:昆明学院,农学院,云南,昆明,650213;昆明学院,农学院,云南,昆明,650213
摘    要:用饱满的微型观赏石榴种子为材料,以MS为基础培养基,添加不同浓度植物生长调节剂(NAA、6-BA)对种子进行无菌萌发试验,并对形成的愈伤组织及试管植株进行快速增殖和生根研究。结果表明:微型观赏石榴种子无菌萌发的适宜配方为MS+6-BA 3.0mg/L+NAA 1.0mg/L+AC 0.3mg/L;最佳增殖配方为MS+6-BA 1.5mg/L+KT 1.5mg/L+NAA 0.5mg/L;最佳生根配方为MS+6-BA 0.5mg/L+IBA 1.5~2.0mg/L+NAA 1.5~2.0mg/L。

关 键 词:微型观赏石榴  组培  种子无菌萌发  增殖  生根

Tissue Rapid Propagation Technique of Tiny Ornamental Pomegranate
WANG Yun-bo,ZHENG Pu-hong. Tissue Rapid Propagation Technique of Tiny Ornamental Pomegranate[J]. Northern Horticulture, 2011, 0(3): 153-155
Authors:WANG Yun-bo  ZHENG Pu-hong
Affiliation:WANG Yun-bo,ZHENG Pu-hong (Kunming Agricultural College,Kunming,Yunnan 650213)
Abstract:Used flush tiny pomegranate seeds as materials,took MS as basic medium,and added with different concentration of plant growth regulators(NAA,6-BA) to test sterile germination,carried out rapid proliferation and rooting study on callus formation.The results showed that the suitable formula for ornamental pomegranate seed germination was MS+6-BA 3.0 mg/L+NAA 1.0 mg/L+AC 0.3 mg/L;the best formula for multiplication was MS+ 6-BA 1.5 mg/L+KT 1.5 mg/L+NAA 0.5 mg/L;the best formula for rooting was MS+6-BA 0.5 mg/L...
Keywords:tiny ornamental pomegranate  tissue culture  sterile seed germination  multiplication  rooting  
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