| 大花萱草工厂化快繁技术研究 |
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| 引用本文： | 赵玉芬,储博彦,尹新彦,刘满光,王丽. 大花萱草工厂化快繁技术研究[J]. 北方园艺, 2011, 0(2): 152-155 |
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| 作者姓名： | 赵玉芬 储博彦 尹新彦 刘满光 王丽 |
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| 作者单位： | 河北省林业科学研究院,河北,石家庄,050061;河北省林木良种工程技术中心,河北,石家庄,050061;保定职业技术学院,河北,保定,071051 |
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| 基金项目： | 河北省林业局重点资助项目 |
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| 摘 要： | 以大花萱草"红运"的茎段和花蕾为外植体,进行了消毒剂和移栽基质的筛选,研究不同浓度激素组合对茎段、花蕾、丛芽愈伤组织诱导和生根的影响。结果表明:茎段愈伤诱导最佳培养基为MS+6-BA 1.5 mg/L+IBA 0.2 mg/L;花蕾愈伤诱导最佳培养基为MS+6-BA 2.0～3.0 mg/L+IBA 0.2 mg/L;丛芽分化增殖适宜的培养基为MS+6-BA 0.3 mg/L+IBA 0.1 mg/L和MS+6-BA 1.5 mg/L+IBA 0.2 mg/L,二者可交替使用;最佳生根培养基为1/2MS+IBA 0.5mg/L;用0.1%HgCl2溶液消毒10 min为宜,蛭石为适宜大规模生产的移栽基质。
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| 关 键 词： | 大花萱草 工厂化育苗 快繁体系 培养基 激素 |
Study on Tissue Culture and Plant Propagation System of Hemerocallis hybrida Hort.cv.Baltim ore oriole |
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| ZHAO Yu-fen,CHU Bo-yan,YIN Xin-yan,LIU Man-guang,WANG Li. Study on Tissue Culture and Plant Propagation System of Hemerocallis hybrida Hort.cv.Baltim ore oriole[J]. Northern Horticulture, 2011, 0(2): 152-155 |
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| Authors: | ZHAO Yu-fen CHU Bo-yan YIN Xin-yan LIU Man-guang WANG Li |
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| Affiliation: | ZHAO Yu-fen~(1,2),CHU Bo-yan~(1,YIN Xin-yan~(1,LIU Man-guang~(1,WANG Li~3 (1.Hebei Academy of Forestry Science,Shijiazhuang.Hebei 050061,2.Hebei Engineering Research Center for Trees Varieties,Shijiazhuang,Hebei 050061,3.Baoding Vocational and Technical College,Baoding,Hebei 071051) |
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| Abstract: | Taking subterranean stem and flower buds of Hemerocallis hybrida Hort.cv.Baltim ore oriole as explants,the disinfectant and transplant material were sifted,the effect of different combinations and concentrations of hormones on the callus induction of stem,flower buds,cluster buds and rooting were studied.The results showed that the best culture medium of subterranean stem was MS+6-BA 1.5 mg/L+IBA 0.2 mg/L,and the best culture medium of flower buds was MS+6-BA 2.0～3.0 mg/L+IBA 0.2 mg/L;for bud proliferation was MS+6-BA 0.3 mg/L + IBA 0.1 mg/L and MS+6-BA 1.5 mg/L +IBA 0.2 mg/L were suitable,and can be used alternately;the best culture medium for rooting was 1/2MS+IBA 0.5 mg/L;In the field,vermiculite was suitable for the transplant material.explants sterilizated with 0.1%HgCl2 for ten minutes were the best sterile method. |
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| Keywords: | Hemerocallis hybrida industrialized nursery process propagation system medium hormone |
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