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| 靶向Annexin A3短发夹环RNA干扰载体的构建及鉴定 | |
| 引用本文： | 席仁荣,刘建军,吴振强,邢秀梅,黄海燕.靶向Annexin A3短发夹环RNA干扰载体的构建及鉴定[J].安徽农业科学,2008,36(17):7145-7146. |
| 作者姓名： | 席仁荣 刘建军 吴振强 邢秀梅 黄海燕 |
| 作者单位： | 1. 深圳市疾病预防控制中心毒理研究室,广东,深圳,518020;华南理工大学生物科学与工程学院,广东,广州,518640 2. 深圳市疾病预防控制中心毒理研究室,广东,深圳,518020 3. 华南理工大学生物科学与工程学院,广东,广州,518640 |
| 基金项目： | 国家自然科学基金资助项目 , 广东省自然科学基金资助项目 |
| 摘 要： | 目的]利用RNA干扰(RNA interfering,RNAi)技术构建和鉴定膜联蛋白A3(Annexin A3)的真核表达载体,为进一步研究Annexin A3在肝细胞中的功能奠定基础。方法]人工合成3对shRNA序列,退火后定向克隆到真核表达载体psiRNA-hH1neo上,采用酶切和测序法鉴定。结果]质粒酶切及测序鉴定得到的产物与预期的目的基因一致。结论]成功构建膜联蛋白A3靶向的真核表达载体。 |
| 关 键 词： | Annexin A3 psiRNA-hH1neo RNA干扰 真核表达载体 |
| 文章编号： | 0517-6611(2008)17-07145-02 |
| 修稿时间： | 2008年4月7日 |
Construction and Identification of shRNA Eukaryotic Expression Vector Targeting Annexin A3 |
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| XI Ren-rong.Construction and Identification of shRNA Eukaryotic Expression Vector Targeting Annexin A3[J].Journal of Anhui Agricultural Sciences,2008,36(17):7145-7146. | |
| Authors: | XI Ren-rong |
| Abstract: | Objective] The aim of this paper was to construct and identify the siRNA eukaryotic expression vector targeting annexin A3.Method]s Four shRNAs were designed according to the coding sequence of annexin A3 gene,and cloned into the downstream of H1 promoter of psiRNA-hH1-neo.The constructed recombinant was analyzed and identified by Ase Ⅰendonuclease digestion and DNA sequencing.Result]s The constructed psiRNA plasmid digested with Ase Ⅰwas linearized.The sequencing result confirmed that the sequence of inserted fragment was correct.Conclusion] Eukaryotic expression vector of siRNA targeting annexin A3 gene was successfully constructed. |
| Keywords: | Annexin A3 psiRNA-hH1neo siRNA Eukaryotic expression vector |
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