氮源比例和不同碳源对珠美海棠离体叶片再生不定芽的影响

摘要：以珠美海棠（Malus zumi Mats）苗叶片为试材,对影响离体叶片再生的因素进行了研究。结果表明：培养基的成分可以显著地影响叶片再生。在激素组合中,BA浓度变化对再生影响较小,而NAA浓度的变化影响较大。MS培养基氮源中,NO3-与NH4+比例以2∶1最好（2∶1MS,即常规MS）,3∶1MS和4∶1MS次之,1∶1MS最差。4种碳源（果糖、蔗糖、葡萄糖、山梨醇）中,果糖明显优于其它3种。适宜的培养基组成为：MS盐、维生素,BA（2.5 mg/L）+NAA（0.25 mg/L）,果糖（30 g/L）,琼脂8 g/L,pH 5.8。叶片再生率为97%,平均再生芽数为5.6个。对碳源影响再生的可能原因进行了讨论。

Influences of Nitrogen Ratio and Carbon Sources on Adventitious Shoots Regeneration of Malus zumi Leaves In Vitro

Abstracts: Factors that affect regeneration of adventitious shoots of Malus zumi Mats leaves in vitro were examined. The results showed that the changes of NAA concentrations were more responsive than that of BA; different ratios of NO3-:NH4+ in MS medium had different results, 2∶1 was the best, followed by 3∶1, 4∶1 and 1∶1; Fructose improved the regeneration of adventitious shoots significantly among the four tested carbon sources which were fructose, sucrose, glucose and sorbitol; And the optimimum medium was consisted of MS salt and vitamins and supplemented with BA (2.5 mg/L), NAA (0.25 mg/L), fructose (30 g/L), agar (8 g/L), and the pH was 5.8. The action mechanism of carbon sources was discussed in the paper.