Comparison of equine bone marrow-, umbilical cord matrix and amniotic fluid-derived progenitor cells |
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Authors: | Arianna Barbara Lovati Bruna Corradetti Anna Lange Consiglio Camilla Recordati Elisa Bonacina Davide Bizzaro Fausto Cremonesi |
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Institution: | 1.Department of Veterinary Clinical Science, Equine Reproduction Unit,Università degli Studi di Milano,Lodi,Italy;2.Department of Biochemistry, Biology and Genetics,Università Politecnica delle Marche,Ancona,Italy;3.IRCCS Istituto Ortopedico Galeazzi,Cell and Tissue Engineering Laboratory,Milan,Italy;4.Mouse & Animal Pathology Laboratory (MAPLab)—FONDAZIONE FILARETE Viale Ortles 22/4,Milano (MI),Italy |
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Abstract: | The aim of the study was to compare in vitro the stemness features of horse progenitor cells derived from bone marrow (BM-MSCs),
amniotic fluid (AF-MSCs) and umbilical cord matrix (EUC-MSCs). It has been suggested that there may be a stem cell population
within both umbilical cord matrix and amniotic fluid. However, little knowledge exists about the characteristics of these
progenitor cells within these sources in the equine species. This study wanted to investigate an alternative and non-invasive
stem cell source for the equine tissue engineering and to learn more about the properties of these cells for future cell banking.
Bone marrow, umbilical cord and amniotic fluid samples were harvested from different horses. Cells were analyzed for proliferation,
immunocytochemical, stem cell gene expression and multilineage plasticity. BM- and AF-MSCs took similar time to reach confluence
and showed comparable plating efficiency. All cell lines expressed identical stem cell markers and capability to differentiate
towards osteogenic lineage. Almost all cell lines differentiated into the adipogenic lineage as demonstrated by cytochemical
staining, even if no adipose gene expression was detectable for AF-MSCs. AF- and EUC-MSCs showed a limited chondrogenic differentiation
compared with BM-MSCs as demonstrated by histological and biochemical analyses. These findings suggest that AF-MSCs appeared
to be a readily obtainable and highly proliferative cell line from an uninvasive source that may represent a good model system
for stem cell biology. More studies are needed to investigate their multilineage potential. EUC-MSCs need to be further investigated
regarding their particular behavior in vitro represented by spheroid formation. |
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