Intraspecific breeding for reduced glucosinolate content in Ethiopian mustard (Brassica carinata A. Braun) |
| |
Authors: | Leonardo Velasco José M Fernández-martínez Antonio De Haro |
| |
Institution: | (1) Apartado 4084, E- 14080, Có |
| |
Abstract: | A total of six Ethiopian mustard ( Brassica carinata A. Braun) lines showing reduced levels of total glucosinolate content
were developed through two different approaches. In the first case, eight lines with good agronomic performance under southern-Spanish
conditions were first evaluated during two years for glucosinolate content, and a pedigree selection was then performed during
three further generations. The line N2-142, with an average glucosinolate content of 82 μmoles g-1 seed, was developed from
the original line C-49, with an average glucosinolate content of 115 μmoles g-1 seed. In the second case, chemical mutagenesis
(EMS 1% v/v) was applied to seeds from the line C- 101, with an average glucosinolate content of 125 μmoles g- 1 seed. Five
mutant lines showing an average glucosinolate content between 20 and 30 μmoles g-1 seed lower than the wild line C-101 were
isolated in the M_3 or M_4 generation. The reduced glucosinolate content of these mutants was confirmed by developing and
analysing the M_5 generation. Previous results in B. juncea suggest that a further reduction of total glucosinolate content
might be achieved through genetic recombination between the different lines developed in this work.
This revised version was published online in July 2006 with corrections to the Cover Date. |
| |
Keywords: | Brassica carinata intraspecific breeding mutagenesis total glucosinolate content |
本文献已被 SpringerLink 等数据库收录! |
|