| 鸡小肠上皮细胞表面大豆凝集素受体的标记 |
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| 引用本文： | 穆成龙,车东升,刘飞飞,王波,张海全,孙泽威,秦贵信. 鸡小肠上皮细胞表面大豆凝集素受体的标记[J]. 吉林农业大学学报, 2011, 33(5): 558-561. DOI: 22-1100/S.20110707.0953.002 |
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| 作者姓名： | 穆成龙 车东升 刘飞飞 王波 张海全 孙泽威 秦贵信 |
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| 作者单位： | 吉林农业大学动物生产及产品质量安全教育部重点实验室,长春130118;吉林农业大学动物科学技术学院,长春130118 |
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| 基金项目： | 国家自然科学基金面上项目(30871802); 吉林省重大科技攻关项目(09ZDGG008) |
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| 摘 要： | 以0日龄海蓝褐蛋鸡为材料,用胶原酶结合组织块培养的方法分离培养了鸡小肠上皮细胞,通过抗角蛋白单克隆抗体对分离的细胞进行纯度鉴定,并用荧光标记大豆凝集素对已鉴定的细胞大豆凝集素结合位点进行了标记.结果表明:试验获得了鸡小肠上皮细胞原代培养物纯度＞90%,可用于后续试验,荧光标记表明细胞表面存在大量的大豆凝集素受体.
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| 关 键 词： | 大豆凝集素 鸡 小肠上皮细胞 荧光标记 |
Labeling of Soybean Agglutinin Binding Sites on Intestinal Epithelial Cells of Chicken |
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| MU Cheng-long,CHE Dong-sheng,LIU Fei-fei,WANG Bo,ZHANG Hai-quan,SUN Ze-wei,QIN Gui-xin. Labeling of Soybean Agglutinin Binding Sites on Intestinal Epithelial Cells of Chicken[J]. Journal of Jilin Agricultural University, 2011, 33(5): 558-561. DOI: 22-1100/S.20110707.0953.002 |
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| Authors: | MU Cheng-long CHE Dong-sheng LIU Fei-fei WANG Bo ZHANG Hai-quan SUN Ze-wei QIN Gui-xin |
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| Affiliation: | MU Cheng-long1,2,CHE Dong-sheng1,LIU Fei-fei1,WANG Bo1,ZHANG Hai-quan1,SUN Ze-wei1,QIN Gui-xin1,21.Animal Production & Product Quality and Security Key Lab,Ministry of Education,Jilin Agricultural University,Changchun 130118,China,2.College of Animal Science and Technology |
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| Abstract: | In this study,intestinal epithelial cells of chicken were isolated and cultured from 0 d old Hy-line variety brown chicken.The cells were identified by the detection of anti-cytokeratin monoclonal antibodies,and the SBA binding sites were detected by using FITC-SBA.The result: the result of anti-cytokeratin detection is positive and the intestinal epithelial cells of chicken with 90% purity were obtained,and FITC-SBA can bind on the surface of the cells. |
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| Keywords: | SBA chicken intestinal epithelial cell fluorescence labeling |
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