| 文冠果离体培养技术研究 |
| |
| 引用本文： | 任皎,芦静,李岩,段加红. 文冠果离体培养技术研究[J]. 吉林林业科技, 2011, 0(6): 13-16 |
| |
| 作者姓名： | 任皎 芦静 李岩 段加红 |
| |
| 作者单位： | 吉林省林业科学研究院;吉林省木材运输管理办公室;中国吉林森林工业集团有限责任公司; |
| |
| 摘 要： | 以文冠果带芽茎段为外植体,以MS配方为基本培养基,采用不同激素组合对其进行离体培养试验,结果表明:6-BA1.00 mg·L-1+NAA0.20 mg·L-1和6-BA1.00 mg·L-1+NAA0.50 mg·L-1的激素组合适宜诱导芽和增殖培养,分化率分别为90.4%和89.3%;苗高分别为1.96 cm和1.9...
|
| 关 键 词： | 文冠果 组织培养 生物调节剂 |
Research on the technology of in vitro culture of Xanthoceras sorbifolia |
| |
| REN Jiao,LU Jing,LI Yan,DUN Jia-hong. Research on the technology of in vitro culture of Xanthoceras sorbifolia[J]. Jilin Forestry Scicnce and Technology, 2011, 0(6): 13-16 |
| |
| Authors: | REN Jiao LU Jing LI Yan DUN Jia-hong |
| |
| Affiliation: | REN Jiao1,LU Jing2,LI Yan1,DUN Jia-hong3(1.Jilin Provincial Academy of Forestry Sciences,Changchun 130033,China,2.Transportation Management Office of Jilin Province,Changchun 130022,3.China Jilin Forest Industry Group Co.,Ltd,Changchun 130021,China) |
| |
| Abstract: | Experiments of in vitro culture were conducted under different hormone combination conditions with MS as basic medium and stems with buds of Xanthoceras sorbifolia as explants.The hormone combinations of MS＋6-BA1.00 mg·L-1＋NAA0.20 mg·L-1 and MS＋6-BA1.00 mg·L-1＋NAA 0.50 mg·L-1 of were more suitable for induction（90.4% and 89.3%,respectively） and proliferation（4.5 and 4.8,respectively） of X.sorbifolia and the seedling heights were 1.96 cm and 1.97 cm,respectively.The 1/2MS＋IBA0.50 mg·L-1 was the optimal rooting medium,with 93.1% rooting rate,4.8 roots and 1.5 cm root length on average.The temperature and humidity should be restrictedly controlled when seedlings were transplanted.Considering the cost and survival rate,the optimal medium for transplantation was garden soilfurnace ash at 21.After transplanted 30 d,the average height of seedlings was 4.5 cm with above 90% survival rate. |
| |
| Keywords: | Xanthoceras sorbifolia issue ulture bio-regulators |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
