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| DP -305423转基因大豆PCR检测方法研究 | |
| 引用本文： | 吕智超,李飞武,周宁,闫伟,王丕武,张明. DP -305423转基因大豆PCR检测方法研究[J]. 吉林农业大学学报, 2011, 33(5): 518-521,526. DOI: 22-1100/S.20110708.0947.001 |
| 作者姓名： | 吕智超 李飞武 周宁 闫伟 王丕武 张明 |
| 作者单位： | 1. 吉林农业大学生命科学学院,长春130118;吉林省农业科学院,长春130033 2. 吉林省农业科学院,长春,130033 3. 吉林农业大学生命科学学院,长春,130118 |
| 基金项目： | 国家转基因生物新品种培育重大专项(2008ZX08011-003) |
| 摘 要： | 根据DP- 305423外源插入片段与植物基因组序列设计特异性引物,以leetin基因(118 bp)作为内参照基因,筛选最佳引物并对反应程序和反应体系进行优化,最终建立转基因大豆DP - 305423转化体特异性定性PCR检测方法.对该方法进行了特异性、灵敏度、稳定性和重复性测试.结果表明:该方法能够特异性检测出DP... |
| 关 键 词： | DP- 305423转基因大豆 定性PCR 检测方法 |
Establishment of an Event-Specific Qualitative PCR Method for Detecting DP-305423 Transgenic Soybean |
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| L Zhi-chao,LI Fei-wu,ZHOU Ning,YAN Wei,WANG Pi-wu,ZHANG Ming. Establishment of an Event-Specific Qualitative PCR Method for Detecting DP-305423 Transgenic Soybean[J]. Journal of Jilin Agricultural University, 2011, 33(5): 518-521,526. DOI: 22-1100/S.20110708.0947.001 | |
| Authors: | L Zhi-chao LI Fei-wu ZHOU Ning YAN Wei WANG Pi-wu ZHANG Ming |
| Affiliation: | LV Zhi-chao1,2,LI Fei-wu2,ZHOU Ning1,YAN Wei2,WANG Pi-wu1,ZHANG Ming21.College of Life Science,Jilin Agricultural University,Changchun 130118,China,2.Jilin Academy of Agricultural Sciences,Changchun 130033 |
| Abstract: | According to DP-305423 exogenous insert sequences connection with the plant genome sequences,the lectin gene was chosen as the reference gene,the best specific primers were designed,the response procedures and response system were optimized,and finally an event-specific qualitative PCR method for detecting DP-305423 transgenic soybean was established.Specificity,sensitivity,stability and repeatability of this method were tested.The results showed that the method can specifically detect soybean DP-305423;DP-... |
| Keywords: | DP-305423 transgenic soybean event-specific PCR detection method |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |