Epidemiology of typical and atypical rotavirus infections in New Zealand pigs |
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| Authors: | ZF Fu DK Blackmore DJ Hampson CR Wilks |
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| Institution: | 1. Department of Veterinary Pathology and Public Health , Massey University , Palmerston North, New Zealand;2. Division of Veterinary Biology , School of Veterinary Studies, Murdoch University , Murdoch, Western Australia , 6150 |
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| Abstract: | Polyacrylamide gel electrophoresis (PAGE) and enzyme-linked immunosorbent assay (ELISA) were employed to investigate the epidemiology of typical and atypical rotavirus infections in five piggeries. Of 152 faecal samples examined, 46 (30 per cent) were positive by ELISA for group A rotavirus. Rotaviruses with electrophoretic patterns resembling groups A, B and C were detected. At least two and up to five different rotavirus electrophoretypes (typical and/or atypical) were detected in each of the five piggeries. Out of 152 faecal samples examined, 28 (18 per cent) contained rotaviruses with group A electrophor etypes, 9 (6 per cent) with group C but only 1 with Group B. Six samples contained both group A and group C rotaviruses. No common electrophoretypes of group A or C rotaviruses were detected in these five piggeries. The PAGE technique was also used to analyze group A rotavirus isolated sequentially from another piggery over a three year period. A singIe electrophoretype was found during the first two years, but in the third year a different electrophoretype was detected. |
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| Keywords: | Canine adenovirus haemagglutination inhibition serum neutralisation restriction endonuclease analysis |
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