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Papular stomatitis of cattle
Authors:R.D. Jolly B.V.Sc.  R.C.W. Daniel B.V.Sc. M.Sc.
Affiliation:Faculty of Veterinary Science, Massey University , Palmerston North
Abstract:A simple method using tissue extraction of Copper (Cu) in 8M nitric acid for 45 minutes at 100°C, followed by sample dilution and Cu quantitation by flame atomic absorption spectroscopy is described. The detection limit of the method is 16 μmol Cu/kg liver. The mean recovery of added Cu was 100.4%. This method for liver copper analysis correlated well with a method using nitric/perchloric acid digestion and atomic absorption spectroscopy (r2 = .986) and is faster, less expensive and less hazardous.
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