The effects of Leptospira serotype pomona in sheep of different haemoglobin types |
| |
| Authors: | R.T. Hodges Ph.D. B.V.Sc. M.R.C.V.S. M.A.C.V.Sc. K.R. Millar Ph.D. M.Sc. K.J.A. Revfeim Ph.D. M.Sc. Dip Math.Stat. |
| |
| Affiliation: | 1. Wallaceville Animal Research Centre , Ministry of Agriculture and Fisheries , Private Bag, Upper Hutt;2. Biometrics Section , Ministry of Agriculture and Fisheries , P.O. Box 2298, Wellington |
| |
| Abstract: | A warm complement fixation test that will detect antibody in sheepserum in the virtual absence of anti-complementary activity is described. Sheep antibody-antigen complexes were detected by fixation of sheep complement. Sheep serum, heparinized or Mg++—EGTA plasma was used as the source of sheep complement. Sheep-antibody-sensitized human erythrocytes were used as the haemolytic indicator cells for sheep complement. As the modified complement-fixation test was performed in the presence of Mg++—EGTA, sheep C probably reacts with sheep Ab-Ag complexes by a different mechanism than does guinea-pig complement. |
| |
| Keywords: | |
|
|
