| 甜椒抗菌蛋白基因(harp)转化桉树的研究 |
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| 引用本文： | 王水琦,陈坚,甘勇辉. 甜椒抗菌蛋白基因(harp)转化桉树的研究[J]. 中南林业科技大学学报(自然科学版), 2007, 27(6): 115-118 |
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| 作者姓名： | 王水琦 陈坚 甘勇辉 |
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| 作者单位： | 漳州职业技术学院食品与生物工程系 福建漳州363000(王水琦，甘勇辉)，福建省农业科学院生物技术研究所 福建福州350003(陈坚) |
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| 基金项目： | 福建省人事厅及福建省科技计划 |
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| 摘 要： | 用不同浓度的2,4-D与IAA对桉树叶盘进行了愈伤组织的诱导及植株再生的试验,建立了新的桉树再生体系.进一步用含甜椒抗菌基因(hrap)的农杆菌侵染桉树叶盘,发现侵染后对愈伤组织诱导率无明显影响,却抑制了桉树的植株再生.对再生苗的PCR和Sou thern杂交检测证实已将hrap基因已转入桉树植株的基因组中.
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| 关 键 词： | hrap基因 抗病基因 桉树 基因转入 组织培养 植株再生 |
| 文章编号： | 1673-923X（2007）06-0115-04 |
| 收稿时间： | 2007-04-17 |
| 修稿时间： | 2007-04-17 |
Studies of Transformation of hrap Gene from Sweet Pepper into Eucalyptus |
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| WANG Shui-qi,CHEN Jian,GANG Yong-hui. Studies of Transformation of hrap Gene from Sweet Pepper into Eucalyptus[J]. , 2007, 27(6): 115-118 |
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| Authors: | WANG Shui-qi CHEN Jian GANG Yong-hui |
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| Abstract: | After a treatment of 2,4-D and IAA with different concentrations,callus was induced from leaf disco of Eucalyptus for regenerating plantlets and a new regeneration system of Eucalyptus was established.Furthermore,the leaf disco of Eucalyptus was infected by Agrobacterium tumefaciens carrying the hrap gene from sweet pepper and no obvious influence was found on the induction rate of callus after the infection,but the regeneration of callus was inhibited.The PCR test and Southern blot of regenerated plantlets show that the hrap gene has incorporated into the genome of Eucalyptus. |
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| Keywords: | hrap gene resistance gene Eucalyptus urophella transgenosis tissue culture plant regeneration |
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