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| 半定量RT-PCR方法研究microRNA393在旱处理后的孕穗期水稻叶片和穗部的表达情况 | |
| 引用本文： | 王迪,傅彬英,张立军.半定量RT-PCR方法研究microRNA393在旱处理后的孕穗期水稻叶片和穗部的表达情况[J].分子植物育种,2008,6(2):355-357. |
| 作者姓名： | 王迪 傅彬英 张立军 |
| 作者单位： | 1. 沈阳农业大学生物科学技术学院,沈阳,110161;中国农业科学院作物科学研究所,北京,100081 2. 中国农业科学院作物科学研究所,北京,100081 3. 沈阳农业大学生物科学技术学院,沈阳,110161 |
| 基金项目： | 国家重点基础研究发展计划(973计划) |
| 摘 要： | microRNAs是新近发现的一类具有重要功能的小分子RNA.研究表明,一些miRNAs与植物抗逆反应相关.本文使用半定量RT-PCR的方法,研究了miR393在旱处理后的孕穗期水稻叶片和穗部的表达情况.结果发现,miR393在胁迫后的叶片和穗部表达上调.同时也发现,miR393在胁迫前后穗部的表达略高于其在叶片中的表达.实验结果也表明,半定量RT-PCR适用于分析miRNAs的表达. |
| 关 键 词： | 半定量RT-PCR miR393 水稻 旱胁迫 半定量 方法 研究 处理 孕穗期 水稻叶片 穗部 表达上调 情况 Drought Stress Rice Booting Stage Leaves 分析 实验 胁迫 结果 使用 相关 逆反应 |
Expression of miR393 in Leaves and Panicles at the Booting Stage of Rice under Drought Stress with Semi-Quantitative RT-PCR |
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| Wang Di,Fu Binying,Zhang Lijun.Expression of miR393 in Leaves and Panicles at the Booting Stage of Rice under Drought Stress with Semi-Quantitative RT-PCR[J].Molecular Plant Breeding,2008,6(2):355-357. | |
| Authors: | Wang Di Fu Binying Zhang Lijun |
| Institution: | Wang Di 1,2 Fu Binying 2 Zhang Lijun 1 1 Biological Science , Technology College,Shenyang Agricultural University,Shenyang,110161,2 Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing,100081 |
| Abstract: | MicroRNAs (miRNAs) are a class of small regulatory RNAs, which involve in plant response to a variety of abiotic and biotic environmental stresses. In this study, we use Semi-Quantitative RT-PCR to detect the expression of miR393. The results showed miR393 was induced by drought and the induction of miR393 was more prominent in panicles than in leaves. The results also indicated Semi-Quantitative RT-PCR was the same with studing the expression of miRNAs. |
| Keywords: | Semi-Quantitative RT-PCR miR393 Rice Drought stress |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |