Seizure activity in dogs is associated with enhanced TIMP-2 expression of microglia |
| |
Authors: | Stein Veronika M Genini Sem Puff Christina Baumgärtner Wolfgang Tipold Andrea |
| |
Institution: | Department of Small Animal Medicine and Surgery, University of Veterinary Medicine, Bünteweg 9, 30559 Hannover, Germany. veronika.stein@tiho-hannover.de |
| |
Abstract: | In the pathogenesis of epilepsy aberrant synaptic plasticity plays an important role. Matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) are responsible for nervous tissue remodelling resulting in synaptic plasticity in the central nervous system (CNS) and might therefore be crucially involved in epileptogenesis. To assess the potential pathogenetic role of microglial MMPs and TIMPs in seizure induction, twenty-four dogs suffering from different intracranial diseases with and without seizure activity were comparatively examined. Microglial cells were isolated by density gradient centrifugation and their expression profiles of MMP-2, MMP-9, MMP-12, MMP-13, MMP-14, TIMP-1, TIMP-2, and RECK (reversion-inducing cysteine-rich protein with Kazal motifs) were examined via quantitative real-time PCR (qPCR). Interestingly, a significant up-regulation of TIMP-2 expression was found for the first time in dogs suffering from seizures. In conclusion, microglial TIMP expression might be involved in seizure generation. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|