日粮添加维生素D3对围产奶牛外周血免疫球蛋白及T细胞亚群的影响

选用16头胎次接近的围产期奶牛,采用完全随机试验设计,研究日粮中额外添加维生素D3(10万IU/(d.头))对围产期奶牛血常规、免疫球蛋白和T细胞亚群CD4 、CD8 的影响。结果表明在日粮中添加VD3可以极显著提高外周血中红细胞、白细胞、血红蛋白含量(P<0.01)。与对照组相比,添加VD3提高血小板数目、淋巴细胞百分比、单核细胞百分比、中性粒细胞绝对值和单核细胞绝对值,但差异不显著(P>0.05)。试验组IgGI、gM和IgA与对照组相比有升高趋势,但差异不显著(P>0.05);试验组细胞亚群的数量均极显著高于对照组(P<0.01)。结果提示,日粮中添加VD3可以缓解围产期奶牛免疫球蛋白和免疫细胞下降的现象。

The Effect of Vitamin D on the Immunoglobulins and T Cell Subsets of Peripheral Blood in Prepartum Dairy Cattle

Sixty Holstein breed cows were randomly assigned to Vitamin D3 or control prepartum diet to investigate the effect of Vitamin D on blood routine,immunoglobulins(Ig),T helper(CD4 ) and T-cytotoxic/suppressor(CD8 ) of peripheral blood.The results showed that RBC,WBC,HGB were increased by supplement VD3 in diet of cow,and the difference is significant(P<0.01).Compared to control group,the PLT,LY(%),MO(%),number of GR,number of MO also were enhanced,but the difference is not significant(P>0.05).At the same time,the IgG,IgM and IgA were higher than control,but the difference is not significant(P>0.05).However,CD4 ,CD8 T cells in Vitamin D treatment were significantly higher than in control group(P<0.01).Ig and T cell subsets decreased in prepartum dairy cattle can be mitigated by supplement VD3 in diets.