| 母羊血清体液免疫干扰因子的ELISA与超早期妊娠诊断 |
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| 引用本文： | 李学斌,杨利国.母羊血清体液免疫干扰因子的ELISA与超早期妊娠诊断[J].中国畜牧杂志,1993,29(6):8-10. |
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| 作者姓名： | 李学斌 杨利国 |
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| 作者单位： | 南京农业大学畜牧系繁殖教研组 210014
(李学斌，杨利国)，南京农业大学畜牧系繁殖教研组 210014(李宁) |
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| 摘 要： | 本研究应用ELISA法测定绵羊血清中干扰体液免疫反应的因子。发现,在配种后48小时,妊娠绵羊血清中干扰体液免疫反应的因子水平显著低于空怀羊。以该因子的配种相对值作为超早期妊娠诊断的依据,可在母羊配种后48小时诊断其是否受胎。判断妊娠和空怀的准确性分别达87.5％和84.3％。
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| 关 键 词： | FIHIR ELISA 超早期妊娠诊断 母羊 |
The Enzyme Linked Immuno-sorbent Assay of Factors Interfering Humoral Immune Response and Its Application for Super-early Pregnancy Diagnosis |
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| Li Xue-bin.The Enzyme Linked Immuno-sorbent Assay of Factors Interfering Humoral Immune Response and Its Application for Super-early Pregnancy Diagnosis[J].Chinese Journal of Animal Science,1993,29(6):8-10. |
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| Authors: | Li Xue-bin |
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| Abstract: | Factors interfering humoral immune response(FIHIR) were detected by means of enzyme linked immunosorbent assay (ELISA) in the serum of ewes. The results showed that the average FIHIR level in serum of pregnant ewes was lower than that of non-pregnant animals at 48 hr after service. The relative values of FIHIR at 48 hr after service can be taken as a reference for super-early pregnancy diagnosis. The reliabilities of diagnoses for pregnancy and non-pregnancy were 87. 5 % and 84. 3% respectively. |
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| Keywords: | FIHI、ELISA、Super-early pregnancy diagnosis、ewes |
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