| 哈密瓜ACC合成酶基因cDNA的克隆及全序列分析 |
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| 引用本文： | 丁群英,张瑞,廖新福,郭蔼光.哈密瓜ACC合成酶基因cDNA的克隆及全序列分析[J].园艺学报,2009,36(8):1177-1183. |
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| 作者姓名： | 丁群英 张瑞 廖新福 郭蔼光 |
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| 作者单位： | (西北农林科技大学生命科学学院, 陕西杨凌712100; 新疆维吾尔自治区葡萄瓜果开发研究中心, 新疆鄯善838201; 西北农林科技大学陕西省农业分子生物学实验室, 陕西杨凌712100) |
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| 基金项目： | 新疆维吾尔自治医自然科学基金 |
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| 摘 要： | 以新疆哈密瓜(Cucumis melo L. ) 幼叶为材料, 根据已报道的甜瓜ACC合成酶3(1-aminocyclopropane-1-carboxylic acid synthase, ACS) 基因片段设计特异引物, 采用RT-PCR和RACE (Rapid Amplification cDNA Ends, cDNA末端快速扩增) 等技术, 克隆得到编码哈密瓜CM e2ACS3基因的全长cDNA 序列,长1 895 bp, 编码482个氨基酸, 分子量约为54.12 kD, 等电点8.45, GenBank登录号为FJ383171。利用软件MEGA4构建进化树, 发现哈密瓜ACS3基因与黄瓜中的同类基因亲缘关系最近。哈密瓜CMe-ACS3基因的克隆及序列分析为认识此基因在甜瓜中的功能研究奠定基础。
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| 关 键 词： | 哈密瓜 ACC合成酶 RACE 序列分析 |
| 收稿时间： | 2009-4-20 |
| 修稿时间： | 2009-6-23 |
Cloning and Sequence Analysis of cDNA Encoding for ACC Synthase from Hami Melon |
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| DING Qun-ying,ZHANG Rui,LIAO Xin-fu,GUO Ai-guang.Cloning and Sequence Analysis of cDNA Encoding for ACC Synthase from Hami Melon[J].Acta Horticulturae Sinica,2009,36(8):1177-1183. |
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| Authors: | DING Qun-ying ZHANG Rui LIAO Xin-fu GUO Ai-guang |
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| Institution: | (College of Life Science, NorthwestA & F University, Yangling, Shaanxi 712100, China; Xinjiang Developm ent and Research Center of Grapes and Melons, Shanshan, Xinjiang 838201, China; Shaanxi Key Laboratory of Molecular Biology forAgriculture,Northwest A & F University, Yangling, Shaanxi 712100, China) |
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| Abstract: | Specific primers were designed based on the reported partial sequence of melon (Cucumis melo L. ) ACS3 gene in the GenBank to clone the full-length cDNA of ACS3 gene in using RT-PCR and RACE methods. Its GenBank Accession No. is FJ383171. The length of Hamimelon ACS gene cDNA is 1 895 bp,this gene encodes 482 amino acids, the putative molecularweight is 54.12 kD and its pI is 8.45. The phylogenetic trees constructed by software MEGA4 showed that ACS3 gene of hamimelon was closely related to that of Cucumis sativus. The cloning of ACS3 gene full-length cDNA from the Hami melon provides the basis for understanding the role of ACS3 gene in melon. |
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| Keywords: | RACE |
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