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Construction of integrated linkage map of a recombinant inbred line population of white lupin (Lupinus albus L.)
Authors:Cina Ann Vipin  David J Luckett  John DI Harper  Gavin J Ash  Andrzej Kilian  Simon R Ellwood  Huyen TT Phan  Harsh Raman
Abstract:We report the development of a Diversity Arrays Technology (DArT) marker panel and its utilisation in the development of an integrated genetic linkage map of white lupin (Lupinus albus L.) using an F8 recombinant inbred line population derived from Kiev Mutant/P27174. One hundred and thirty-six DArT markers were merged into the first genetic linkage map composed of 220 amplified fragment length polymorphisms (AFLPs) and 105 genic markers. The integrated map consists of 38 linkage groups of 441 markers and spans a total length of 2,169 cM, with an average interval size of 4.6 cM. The DArT markers exhibited good genome coverage and were associated with previously identified genic and AFLP markers linked with quantitative trait loci for anthracnose resistance, flowering time and alkaloid content. The improved genetic linkage map of white lupin will aid in the identification of markers for traits of interest and future syntenic studies.
Keywords:Lupinus albus  genetic linkage mapping  molecular markers  Diversity Array Technology
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