3种地锦草总黄酮和鞣质质量分数及体外抗菌活性比较

采用紫外-可见分光光度法测定陕西产地锦、斑地锦、小叶地锦总黄酮和鞣质质量分数,应用微量肉汤稀释法测定其在体外对大肠埃希菌、鼠伤寒沙门菌、金黄色葡萄球菌、无乳链球菌的最小抑菌质量浓度(MIC)和最小杀菌质量浓度(MBC)。结果表明,地锦、斑地锦、小叶地锦的总黄酮质量分数分别为16.53、18.62、22.65mg/g,鞣质质量分数分别为38.31、32.78、41.44mg/g。地锦、斑地锦、小叶地锦对大肠埃希菌MIC、MBC均为125g/L,对鼠伤寒沙门菌MIC分别为62.5、31.25、62.5g/L,MBC分别为125、62.5、250g/L,对金黄色葡萄球菌MIC分别为125、31.25、62.5g/L,MBC分别为250、125、62.5g/L,对无乳链球菌MIC分别为125、62.5、125g/L,MBC分别为125、250、125g/L。陕西产斑地锦,尤其是小叶地锦为中药地锦草的潜在新基原。

Comparison on Mass Fraction of Total Flavonoids and Tannin of Three Kinds of Herba Euphorbiae Humifusae and Their Antibacaterial Activity in vitro

To compare the mass fraction of total flavonnoids and tannin of three kinds of Herba Euphorbia humifusa and their antibacaterial activity in vitro, the total flavonoids and tannin of Euphorbia humifusa Willd, E. maculata L. and E. heyneana Spreng from Shaanxi province were determined by UV-Vis spectrophotometry, and their minimal inhibitory concentration (MIC) and minimal bactericidal concentrations (MBC) against Escherichia coli, Salmonella typhimurium, Staphylococcus aureus and Streptococcus agalactiae in vitro were determined by broth microdilution method. The result showed that the mass fraction of total flavonoids of E. humifusa, E. maculata and E. heyneana was 16.53,18.62,22.65 mg/g, respectively, and the mass fraction of tannin was 38.31,32.78,41.44 mg/g, respectively. Against Escherichia coli, their MIC, MBC all were 125 g/L. Against Salmonella typhimurium,MIC was 62.5,31.25,62.5 g/L,and MBC was 125,62.5,250 g/L,respectively.Against Staphylococcus aureus,MIC was 125,31.25,62.5 g/L,and MBC was 250,125,62.5 g/L,respectively. Against Streptococcus agalactiae,MIC was 125,62.5,125 g/L,and MBC was 125,250,125 g/L,respectively. The plant of E. maculate, especially, E. heyneana from Shaanxi province is a new potential origin.