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猪DECR1基因SNPs筛选及其多态性分析
引用本文:黄生强,柳小春,蒋隽,马乐. 猪DECR1基因SNPs筛选及其多态性分析[J]. 湖南农业大学学报(自然科学版), 2011, 37(2): 192-194. DOI: 10.3724/SP.J.1238.2011.00192
作者姓名:黄生强  柳小春  蒋隽  马乐
作者单位:湖南农业大学动物科学技术学院,湖南长沙,410128
基金项目:国家"863"项目,湖南省教育厅项目
摘    要:以大白猪、长白猪和杜洛克3个猪种共327头为试验材料,对2,4-dienoyl-CoA reductase 1(DECR1)基因第6至第10外显子区域进行SNPs筛选,分析SNP突变位点的遗传多态性;采用比较基因组方法,根据人DECR1基因全长序列设计引物,筛选猪DECR1基因的SNP,经PCR-SSCP技术和测序检测...

关 键 词:  单核苷酸多态性  DECR1基因  遗传多态性
收稿时间:2010-12-20
修稿时间:2011-03-08

SNPs of DECR1 gene and its genetic polymorphism in pigs
huang sheng qiang,liu xiao chun,jiang juan and ma le. SNPs of DECR1 gene and its genetic polymorphism in pigs[J]. Journal of Hunan Agricultural University, 2011, 37(2): 192-194. DOI: 10.3724/SP.J.1238.2011.00192
Authors:huang sheng qiang  liu xiao chun  jiang juan  ma le
Affiliation:College of Animal Science and Technology;HNAU,College of Animal Science and Technology;HNAU,College of Animal Science and Technology;HNAU,College of Animal Science and Technology;HNAU
Abstract:Three hundred and twenty seven Yorkshire, Landrace and Duroc pigs were analysed to screen the SNPs in 610 Exon of mitochondrial 2,4-dienoyl-CoA reductase 1(DECR1) gene and analyse its genetic structure. The resluts showed: the primers were designed accroding to human DECR1 gene by means of genome comparison, 2 SNPs were screened out in Exon6 and Exon8 by PCR-SSCP method, but mutation in Exon6 loic belonged to nonsense mutation, mutation in Exon8 loci belong to missense mutation. The polymorphism of the SNP in Exon8 were studied and 3 genotypes (CC, CG, GG) were tested in 3 breeds. There were abundant genetic polymorphism in Exon8 loci.
Keywords:pig   single nucleotide polymorphisms(SNP)   DECR1 gene   genetic polymorphism
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