Isolation of a viable but non-culturable suppression mutant of Vibrio vulnificus: role of antioxidant enzymes in surviving stationary phase and low temperatures |
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Authors: | Akihisa Abe Eiji Ohashi Huifeng Ren Tetsuhito Hayashi Hideaki Endo |
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Affiliation: | Nippon Suisan Kaisha Ltd, Food Safety Research Center, Hachioji, Tokyo 192-0906, and;Department of Ocean Science, Tokyo University of Marine Science and Technology, Konan, Minato, Tokyo 108-8477 Japan |
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Abstract: | ABSTRACT: The viable but non-culturable (VBNC) suppression mutant was isolated. Suppression subtractive hybridization was used to identify differentially expressed genes among cDNA of the VBNC suppression mutant and the wild-type strain. RpoS-dependent catalase was highly expressed from the VBNC suppression mutant during oxidative stress in the stationary phase and at low temperatures. In experiments of H2O2 sensitivity, the VBNC suppression mutant showed a peroxide-resistant phenotype in the stationary phase and at low temperatures. These results suggest that VBNC suppression mutant cells use catalase for protection against oxidative stress in the stationary phase and at low temperatures. |
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Keywords: | NTG mutagenesis subtractive hybridization viable but non-culturable Vibrio vulnificus |
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