青叶胆种子萌发的研究

[目的]为青叶胆种子的保存及人工驯化栽培提供技术依据。[方法]从云南弥勒山采集青叶胆种子,自然风干20 d后测其千粒重;在不同温度(15、20、25、30、35、40℃)及光照(红、黄、蓝、绿、白光及黑暗)条件下培养30 d后,记录发芽种子数;分别将种子在GA3(100和200 mg/L)溶液、3%KNO3溶液及蒸馏水(CK)中浸泡3 h,在(25±1)℃、自然光照下培养30 d,计算种子发芽率。[结果]青叶胆种子发芽的适宜温度为20~30℃;光照对种子发芽有促进作用;在自然状态下贮藏4个月的种子发芽率最高;种子寿命约为7个月,4℃低温冷藏可延长种子寿命;GA3、KNO3浸种可打破种子休眠,显著提高种子发芽率。[结论]该试验结果与大多数相关研究结果一致。

Study on the Seed Germination of Swertia mileensis

[Objective] The study was to provide the technical basis for preserving and artificial domesticating and cultivating Swertia mileensis seeds.[Method] The seeds of S.mileensis were collected from Minle mountain in Yunnan,and its 1 000-seed weight was measured after natural dry for 20 d.The number of germinating seeds were recorded after being cultivated for 30 d under the conditions of different temperature(15,20,25,30,35,40 ℃) and different illumination(red,yellow,blue,green,white and dark).The seed germination rate was calculated after the seeds being soaked for 3.0 h in GA3(100,200 mg/L GA3 solution),3% KNO3 solution and distilled water(CK) and being cultivated for 30 d under(25±1) ℃ and natural light.[Result] The suitable temperature for germination of S.mileensis seeds was 20-30 ℃.The illumination had promoting effect on seed germination.The seed germination rate was the highest after being stored for 4 months under the condition of natural state.The seed longevity was about 7 months,and the seed longevity could be prolonged by low temperature under 4 ℃.The seed dormancy could be broken,and the seed germination rate could be increased significantly with the method of seed soaking with GA3 and KNO3.[Conclusion] The results of the test were consistent with that of most of related studies.