| 利用RAPD和ISSR标记分析苎麻野生种质资源的遗传多样性 |
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| 引用本文： | 蒙祖庆,刘立军,彭定祥.利用RAPD和ISSR标记分析苎麻野生种质资源的遗传多样性[J].分子植物育种,2009,7(2). |
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| 作者姓名： | 蒙祖庆 刘立军 彭定祥 |
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| 作者单位： | 1. 西藏农牧学院,林芝,860000;华中农业大学植物科学技术学院,武汉,430070
2. 华中农业大学植物科学技术学院,武汉,430070 |
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| 基金项目： | 农业部引进国际先进农业科学技术计划(948计划)，湖北省科技攻关项目 |
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| 摘 要： | 本文以8个地方栽培品种为参照,应用RAPD和ISSR标记从DNA水平分析了来自于不同生态区域的30份苎麻野生种质的遗传背景.在31条RAPD引物中,共扩增出358个条带,平均产率为11.5条带/条引物;而在18对ISSR引物共扩增出266个条带,平均产率为14.8条带/条引物.用NTSYs 2.0软件进行UPGMA法聚类.聚类分析结果表明:在0.73的相似系数水平上,均可将38份材料分成8大类群,对两种标记的比较和混合分析得出:RAPD和ISSR标记适用于苎麻野生材料的遗传多样性分析,但ISSR比RAPD标记更适合苎麻野生种质资源亲缘关系分析.这为我们以后的苎麻杂交育种提供了重要的依据.
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| 关 键 词： | 苎麻野生资源 RAPD和ISSR 多样性分析 |
Analysis on Genetic Diversity of Ramie (Boehmeria nivea L. Gaud) Wild Germplasm by RAPD and ISSR Markers |
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| Meng Zuqing,Liu Lijun,Peng Dingxiang.Analysis on Genetic Diversity of Ramie (Boehmeria nivea L. Gaud) Wild Germplasm by RAPD and ISSR Markers[J].Molecular Plant Breeding,2009,7(2). |
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| Authors: | Meng Zuqing Liu Lijun Peng Dingxiang |
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| Institution: | Meng Zuqing 1,2 Liu Lijun 2 Peng Dingxiang 2 1 Tibet Agricultural , Animal Husb,ry College,Linzhi,860000,2 College of Plant Science , Technology,Huazhong Agricultural University,Wuhan,430070 |
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| Abstract: | 8 local cultivars and 30 wild germplasm accessions were studied by using RAPD and ISSR markers to discuss ramie's molecular background. The result showed 358 strips were amplified by 31 RAPD primers while 266 strips by 18 ISSR primers. The average strips amplified by RAPD and ISSR primers were 11.5 and 14.8, respectively. The genetic relationships were analyzed using unweighted pair-group method of arithmetic average cluster analysis (UPGMA) and the genetic Jaccard similarity coefficients were calculated. T... |
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| Keywords: | Ramie wild germplasm RAPD and ISSR Diversity analysis |
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