The histology and histochemical aspects of gills of the flower fish, <Emphasis Type="Italic">Pseudophoxinus antalyae</Emphasis> |
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Authors: | Kenan Cinar Abdulkerim Aksoy Yilmaz Emre Resat N A?ti |
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Institution: | (1) Department of Biology, Faculty of Science and Art, Süleyman Demirel University, Isparta, Turkey, 32260;(2) Mediterranean Fisheries Research Production and Training Institute, Antalya, Turkey, 07001;(3) Department of Histology and Embryology, Veterinary Faculty, Ankara University, Ankara, Turkey |
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Abstract: | Branchial epithelium of Pseudophoxinus antalyae was lined by both a thick stratified epithelium lining gill arches, gill rakers and primary filaments and a thin epithelium
lining the lamellae. Mucous, chloride and rodlet cells, interspersed between pavement cells, were present in the branchial
epithelium. With histochemical procedures for the characterization of glycoconjugates, mucous cells showed a strong positive
reaction with Periodic acid-Shiff and Alcian Blue at pH 2.5, although with Alcian Blue at pH 0.5 and pH 1.0 the reaction was
much weaker. When the combined Alcian Blue (pH 2.5) – Periodic acid-Shiff reaction was performed, most mucous cells were stained
purple, whereas by the combined Aldehyde Fuchsin/Alcian Blue (pH 2.5), most cells showed a positive reaction only to Aldehyde
Fuchsin. Methylation/ Alcian Blue (pH 2.5) and Methylation/ Saponification/ Alcian Blue (pH 2.5) methods showed the presence
of sulphated and carboxylated glycoconjugates in mucous cells. Mucous cells were also detected to stain all metachromatically
with Toluidine Blue. |
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Keywords: | Pseudophoxinus antalyae Gill Mucous cells Glycoconjugates Histology Histochemistry |
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