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| 猪HSL基因PCR-RFLP多态性研究 | |
| 引用本文： | 吴珍芳,熊远著,邓昌彦,蔡更元,Harbitz. 猪HSL基因PCR-RFLP多态性研究[J]. 华中农业大学学报, 2000, 19(3) |
| 作者姓名： | 吴珍芳 熊远著 邓昌彦 蔡更元 Harbitz |
| 作者单位： | 1. 华中农业大学猪遗传改良农业部重点实验室,武汉,430070 2. 广东农业科学院畜牧研究所,广州,510640 3. 挪威兽医学院,奥斯陆,挪威,8146,N-0033 |
| 基金项目： | TheprojectisgrantedbyDoctoralPositionFoundationfromNationEducationCommittee |
| 摘 要： | 激素敏感脂肪酶 (HSL)是动物体脂肪分解的关键酶。本研究采用PCR RFLP法研究了 5个不同品种猪HSL基因部分 5’端区域和第 6内含子至第 9外显子区域DNA多态性。结果在对应于基因第 7内含子的DNA扩增片段中发现一AluI酶切位点多态性 ,梅山猪和湖北白猪中表现 3种等位基因型 (PP、PQ和QQ) ,等位基因P和Q的频率分别为 0 .6 5、0 .35和 0 .1 5和 0 .85,而通城猪中全部表现为PP基因型 ,大白猪和长白猪中全部表现为QQ基因型 |
| 关 键 词： | 激素敏感脂肪酶 多态性 限制性片段长度多态性(RFLP) 猪 |
Study on the Polymorphism of Porcine Hormone-Sensitive Lipase Gene by PCR-RFLP |
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| Wu Zhenfang Xiong Yuanzhu Deng Changyan Cai Gengyuan I.Harbitz. Study on the Polymorphism of Porcine Hormone-Sensitive Lipase Gene by PCR-RFLP[J]. Journal of Huazhong Agricultural University, 2000, 19(3) | |
| Authors: | Wu Zhenfang Xiong Yuanzhu Deng Changyan Cai Gengyuan I.Harbitz |
| Abstract: | Hormone sensitive lipase (HSL) is a key enzyme in fat metabolism. The polymorphism of the HSL gene was studied in this study. It revealed an AluI-sit e polym orphism in amplified fragments in intron 7 of HSL gene by PCR-RFLP. Th ere exists three genotypes (PP、PQ、QQ) in the Meishan Pigs and the Hubei White Pigs and with allele frequeneies of 0.645P,0.355Q and 0.15P,0.85Q, respectively. Ho wever, the PP genetype was only found in Tongcheng Pigs and QQ genetype in Landr ance and Large White Pigs. |
| Keywords: | hormone-sensitive lipase polymorphism PCR-RFLP pig |
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