| 利用胚和胚珠的离体培养获得甘蓝型油菜与青花菜的种间杂种 |
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| 引用本文： | 陈玉萍,AndrzejWojciechowski. 利用胚和胚珠的离体培养获得甘蓝型油菜与青花菜的种间杂种[J]. 华中农业大学学报, 2000, 19(3) |
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| 作者姓名： | 陈玉萍 AndrzejWojciechowski |
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| 作者单位： | 1. 华中农业大学园艺系,武汉,430070
2. 波兹南农业大学植物遗传育种系,波兹南,波兰60-112 |
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| 摘 要： | 以波兹南农业大学植物遗传育种系提供的甘蓝型胞质雄性不育油菜为母本与青花菜杂交 ,授粉后 1 9d,观察其种间杂交的有效性 ,并对其胚和胚珠进行离体培养。结果表明 :种间杂交的亲和性及胚珠退化时间随杂交组合不同而异 ,亲本的基因型对其有很大的影响。胚和胚珠的离体培养可有效地克服合子后的发育障碍获得种间杂种植株
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| 关 键 词： | 甘蓝型油菜 青花菜 种间杂种 胚和胚珠的离体培养 |
Obtained Interspecific Hybrid of B. napus and B. oleracea var. italica by Embryo and Ovule in vitro culture |
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| Chen Yuping Andrzej Wojciechowski. Obtained Interspecific Hybrid of B. napus and B. oleracea var. italica by Embryo and Ovule in vitro culture[J]. Journal of Huazhong Agricultural University, 2000, 19(3) |
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| Authors: | Chen Yuping Andrzej Wojciechowski |
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| Abstract: | Cytoplasm male sterility Brassica napus supplied by the Department of Genetics and Plant Breeding of Poznan Agricultural University was used as maternal plant to cross with Brassica oleracea var. italica . The interspecific cross effectiveness was observed, embryos and ovules were in vitro cultured at 19 days after pollinating. The results showed that the interspecific cross compatibility and the time of ovule degeneration varied with cross combinations, the genetype had a great influence on crossing. Embryo and ovule in vitro cultures could overcome postzygotic barriers effectively and obtained interspecific hybrids. |
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| Keywords: | Brassica napus B. oleracea var. italica interspecific hybrid embryo and ovule in vitro cultures |
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