Using direct immunofluorescence to detect coronaviruses in peritoneal in peritoneal and pleural effusions |
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| Authors: | M Cammarata Parodi G Cammarata S Paltrinieri A Lavazza† F Ape |
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| Institution: | Istituto di Anatomia Patologica Veterinaria e Patologia Aviare, Via Celoria 10, 20133 Milano, Italy;*Istituto di Patologia Generale Veterinaria, Milano, Italy;?Istituto Zooprofilattico Sperimentale, Brescia, Italy |
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| Abstract: | Twenty-one cases of feline infectious peritonitis (FIP) were diagnosed using a direct immunofluorescence test on cytocentrifuged pleural and peritoneal effusions from cats sampled in vivo (11 cases) and at necropsy (10 cases). A commercial fluorescent polyclonal antiserum of feline origin reacting with FIPV and cross reacting with transmissible gastroenteritis virus and canine coronavirus was used. Eleven cats with ascites of a different origin were used as negative controls. The direct immunofluorescence test was 97 per cent reliable (31 cases of 32) and can be used in routine diagnosis. |
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