| 道地药材怀牛膝根部内生真菌的种群探讨 |
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| 引用本文： | 孙思胜,魏启航,马传贵,张化阁,陈林. 道地药材怀牛膝根部内生真菌的种群探讨[J]. 现代农业科技, 2021, 14(14) |
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| 作者姓名： | 孙思胜 魏启航 马传贵 张化阁 陈林 |
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| 作者单位： | 许昌学院 食品与药学院/河南省食品安全生物标识快检技术重点实验室,许昌学院 食品与药学院/河南省食品安全生物标识快检技术重点实验室,北京京诚生物科技有限公司 北京,舞钢市沁丰园林业专业合作社,黄河科技学院 郑州市天然产物合成生物学重点实验室 |
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| 基金项目： | 河南省科技攻关项目(192102310441,202102110055)。 |
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| 摘 要： | 为探讨道地药材怀牛膝（Achyranthes bidentata Blume）内生真菌的菌群组成及侵染情况。通过组织块分离法，选取野生怀牛膝根部作为分离对象，进行内生真菌分离，并采用形态学和分子生物学两种方法对分离得到的真菌进行鉴定。从怀牛膝根部中共分离纯化获得101 株内生真菌，根据形态学和分子生物学特征，它们属于 6 目 9科 10属；根部内生真菌侵染率高达80%。根部分离得到13种。怀牛膝根部内生真菌表现出丰富的种属多样性。在怀牛膝根部分离的内生真菌中，优势菌属是镰孢属和Moesziomyces。
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| 关 键 词： | 道地药材，怀牛膝 内生真菌 根部 |
| 收稿时间： | 2021-02-01 |
| 修稿时间： | 2021-02-02 |
Study on the species community of Endophytic Fungi in Roots of Achyranthes bidentata Blume |
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| ZHANG Huage and. Study on the species community of Endophytic Fungi in Roots of Achyranthes bidentata Blume[J]. Modern Agricultural Sciences and Technology, 2021, 14(14) |
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| Authors: | ZHANG Huage and |
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| Affiliation: | Qinfengyuan Forestry Professional Cooperatives of Wugang Henan Wugang, |
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| Abstract: | To investigate endophytic fungi species community and colonization in dao-di herbs Achyranthes bidentata Blume, which roots were selected as the objects of isolation by tissue block method. The endophytic fungi were isolated and identified by morphological observation and molecular biological characteristics.101 strains of endophytes were isolated and purified from the roots. According to their morphological and molecular biological characteristics,they belonged to 6 orders,9 families,10 genera,13 species. The colonization rate of endophytic fungi in roots high to71.7% There were diversity endophytic fungi in roots. The most frequently isolated genera were Fusarium and Moesziomyces in the roots. |
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| Keywords: | Dao-di herbs Achyranthes bidentata Blume endophytic fungi root |
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