| Cloning and Analysis of Telomere-associated Sequences of Ginkgo biloba L.. |
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| 作者单位： | . Liu Di Lu Hai Ji Fei-teng Li Feng-lan* Guo Hui-hong College of Biological Sciences and Biotechnology，Beijing Forestry University，Beijing 100083，P. R. China |
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| 摘 要： | Total genomic DNA was extracted from leaves of Ginkgo biloba L. by the method of hot CTAB. After determining quantification of DNA sample by microclorimetric spectrophotography, Arabidopsis-type telomere primer and Sau3AⅠ cassette primer were used to isolate telomere-associated sequences from G. biloba L. by the method of cassette-ligation-mediated polymerase chain reaction (PCR). Using this method, two telomere-associated sequences, TAS1 and TAS2, were isolated. The authors preformed Southern hybridization of EcoRⅠ-treated G. biloba genomic DNA with each clone. The hybridization pattern showed that the clones obtained were derived from G. biloba genomic DNA. There are the Arabidopsis-type TTTAGGG tandem repeats in telomeres of G. biloba.
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Cloning and Analysis of Telomere-associated Sequences of Ginkgo biloba L. |
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| . Liu Di Lu Hai Ji Fei-teng Li Feng-lan Guo Hui-hong College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing ,P. R. China. Cloning and Analysis of Telomere-associated Sequences of Ginkgo biloba L.[J]. Forestry Studies in China, 2005, 7(1) |
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| Authors: | . Liu Di Lu Hai Ji Fei-teng Li Feng-lan Guo Hui-hong College of Biological Sciences Biotechnology Beijing Forestry University Beijing P. R. China |
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| Affiliation: | . Liu Di Lu Hai Ji Fei-teng Li Feng-lan* Guo Hui-hong College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing 100083,P. R. China |
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| Abstract: | |
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| Keywords: | telomere-associated sequences (TASs) cassette-ligation-mediated PCR Ginkgo biloba L. |
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