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高黄酮含量野葛淀粉提取工艺优化
引用本文:何美军,王华,张宇,穆森,郭坤元,何银生.高黄酮含量野葛淀粉提取工艺优化[J].湖北农业科学,2016(24):6537-6540.
作者姓名:何美军  王华  张宇  穆森  郭坤元  何银生
作者单位:湖北省农业科学院中药材研究所,湖北 恩施,445000
基金项目:恩施州科技计划项目(XYJ2016000305)
摘    要:采用单因子和正交试验方法对富含黄酮的野葛(Pueraria lobata)淀粉提取工艺进行了研究,对水、甲醇、乙醇3种溶剂提取效果进行了比较,以淀粉中的总黄酮含量为参考指标,分别对4个提取参数(提取温度、乙醇体积分数、提取时间、固液比)进行单因子试验,然后采用正交试验对提取条件进行优化。结果表明,野葛淀粉高黄酮含量提取的工艺条件为提取温度35℃、乙醇体积分数50%、提取时间3.0 h、固液比1∶8(kg∶L)。

关 键 词:野葛(Pueraria  lobata)  黄酮  淀粉

Optimization of Technology for Extracting Pueraria lobata Starch with High Content of Flavonoids
Abstract:The technology for extracting Pueraria lobata starch with high content of f1avonoids was studied by sing1e factor ex-periment and orthogona1 tests, three kinds of so1vents inc1uding water, methano1, ethano1 were compared, with the content of tota1 f1avonoids in starch as reference index, 4 extraction parameters (extraction temperature, ethano1 concentration, extraction time, so1id-1iquid ratio) were tested by sing1e factor experiment, then the extraction conditions were optimized by orthogona1 tests. The resu1ts showed that the techno1ogica1 conditions for extracting Pueraria lobata starch with high content of f1avonoids:extraction temperature 35℃, ethano1 concentration 50%, extraction time 3.0 h, so1id-1iquid ratio 1:8(kg:L).
Keywords:Pueraria lobata  f1avonoids  starch
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