家禽粪便除臭复合菌剂的应用研究

为研究复合菌剂的除臭机理,将放线菌5406(Actinobacillus)、枯草芽孢杆菌B-903(Bacillus subtilis)、卷枝毛霉(Mucor circinelloides)、钾细菌(Bacillus mucilaginosus)、植物乳杆菌Z3-1(Lactobacillus plantarum)和毕赤酵母(Pichia pastoris)按不同比例配制成复合菌剂,将其均匀喷洒到鸡粪表面并搅拌均匀,进行鸡粪堆肥对比试验,分别筛选出对甲基吲哚、硫化氢、氨气去除效果最好的除臭菌剂配方,并在已筛选出的配方中添加生物炭及化学试剂,检测除臭效果。结果表明,去除甲基吲哚效果最好的除臭菌剂配方为C4组(放线菌5406、枯草芽孢杆菌B-903、卷枝毛霉、钾细菌、植物乳杆菌Z3-1和毕赤酵母各25 m L,生物炭11 g,甲醇0.04 mg);去除氨气效果最好的除臭菌剂配方为D3组(放线菌5406、枯草芽孢杆菌B-903、卷枝毛霉、钾细菌、植物乳杆菌Z3-1和毕赤酵母各20 m L,二价铁离子和抗坏血酸各0.06 g);去除硫化氢效果最好的除臭菌剂配方为E4组(放线菌5406、枯草芽孢杆菌B-903、卷枝毛霉、钾细菌、植物乳杆菌Z3-1和毕赤酵母各20 m L,氧化锌0.08 mg)。

Application of Complex Microbial Deodorization of Poultry Manure

For the deodorization mechanism of composite microbial agent,Actinobacillus 5406,Bacillus subtilis B-903,Mucor circinelloides,Bacillus mucilaginosus,Lactobacillus plantarum Z3-1 and Pichia pastoris were prepared according to different proportion composite agents and evenly sprayed to the surface of chicken manure and stirred.Chicken manure compost contrast test were screened on methyl indole,hydrogen sulfide,ammonia removal deoderizer formula,and biochar and chemical reagents were added in the screened formula,and the deodorization effect was detected.The results showed that the best deoderizer formula of removing methylindole was C4 group (Actinobacillus 5406,Bacillus subtilis B-903,Mucor circinelloides,Bacillus mucilaginosus,Lactobacillus plantarum Z3-1 and Pichia pastoris 25 mL each,biochar 11 g,methanol 0.04 mg);the best deoderizer formula of removing ammonia was D3 group(Actinobacillus 5406,Bacillus subtilis B-903,Mucor circinelloides,Bacillus mucilaginosus,Lactobacillus plantarum Z3-1 and Pichia pastoris 20 mL each,divalent iron ion and ascorbic acid 0.06 g);the best deoderizer formula of removing hydrogen sulfide was E4 group(Actinobacillus 5406,Bacillus subtilis B-903,Mucor circinelloides,Bacillus mucilaginosus,Lactobacillus plantarum Z3-1 and Pichia pastoris 20 mL each,zinc oxide 0.08 mg).