鸡新城疫──减蛋综合症二联油乳剂灭活疫苗研制新工艺及应用 |
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引用本文: | 康昭风,周希平.鸡新城疫──减蛋综合症二联油乳剂灭活疫苗研制新工艺及应用[J].江西农业学报,1999,11(2):10-14. |
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作者姓名: | 康昭风 周希平 |
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摘 要: | 用高压均质机制备的ND-EDS76二联油乳剂灭活疫苗,经3000rpm,10min离心不破乳,37℃恒温保存32d以上未破乳,粘度为3~4Sec;用该油乳剂灭活疫苗接种商品蛋鸡,每羽经肌肉注射0.5mL,接种14d后即可产生足够保护力的中和抗体,2个月时间抗体可达高峰;接种50周后,NDHI抗体效价仍维持在8.2log2,EDS76HI抗体效价7.0log2。在4~8℃冰箱中保存,有效期在一年以上,经80万头份中间试验证明,该疫苗对外来品种鸡和地方品种鸡同样有效,能有效控制ND和EDS76两种疾病。
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关 键 词: | ND-EDS76 油乳剂疫苗 工艺 |
Application and New Technique of Inactivated Vaccine in Oily Adjuvant against ND and EDS 76 |
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Abstract: | The inactivated vaccine in oily adjuvant against ND and EDS 76 was made with high-pressure homogeneous machine.The stabilty of inactivated vaccine was very well. The inactivated vaccine was used to inoculate commercial egg-laying chicken by muscle injecting 0.5mL per hen, and had good immune effect. The HI antibody valence of ND and EDS 76 still kept at 8.21 log2 and 7.01 log2 respectively at 50 weeks after inoculation. |
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Keywords: | NDV EDS 76V Inactivated vaccine Technique |
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