| 橡胶树内生真菌分离方法研究 |
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| 引用本文： | 李晓娜,王 赟,李增平,郑服丛. 橡胶树内生真菌分离方法研究[J]. 热带作物学报, 2009, 29(6): 6-8 |
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| 作者姓名： | 李晓娜 王 赟 李增平 郑服丛 |
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| 作者单位： | 海南大学环境与植物保护学院 海南儋州 571737;海南大学环境与植物保护学院 海南儋州 571737;海南大学环境与植物保护学院 海南儋州 571737;海南大学环境与植物保护学院 海南儋州 571737 |
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| 摘 要： | 以同一株橡胶树的枝条和叶片为材料,采用5种不同的组织表面消毒方法，在加入3种不同细菌生长抑制剂的PDA培养基中培养橡胶树内生真菌。结果表明，在加入Rifamicin 15 mg/L的PDA培养基中，运用M2培养分离效果最佳。
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| 关 键 词： | 橡胶树 ；内生真菌 ；分离方法 |
Separation of Endophytic Fungi in Hevea brasiliensis |
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| Affiliation: | Separation of Endophytic Fungi in Hevea brasiliensis;Separation of Endophytic Fungi in Hevea brasiliensis;Separation of Endophytic Fungi in Hevea brasiliensis;Separation of Endophytic Fungi in Hevea brasiliensis |
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| Abstract: | Leaves and branches from the same rubber tree (Hevea brasiliensis) were collected as materials and sterilized with 5 different sterilization techniques, and cultured in PAD culture medium supplemented with 3 different bacterial growth inhibitors to produce endophytic fungi. The results showed that the leaves and branches sterilized with the M2 method (sterilized with 75% ethanol for 1 min, followed by 4% sodium hypochlorite for 4 min and 75% ethanol for 30 sec, and rinsed with aqua sterilisa) and cultured on the PDA medium added with Rifamicin (15 mg/L) produced the largest number of endophytic fungi. |
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| Keywords: | Hevea brasiliensis endophytic fungi culture medium |
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