| 7株牛源A型巴氏杆菌的分离鉴定及耐药性分析 |
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| 引用本文： | 王梓,马红霞. 7株牛源A型巴氏杆菌的分离鉴定及耐药性分析[J]. 中国兽药杂志, 2015, 49(7): 8-12 |
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| 作者姓名： | 王梓 马红霞 |
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| 作者单位： | 吉林农业大学动物科技学院,吉林农业大学动物科技学院 |
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| 基金项目： | 国家自然科学( 31140026) ; 吉林省教育厅“十二五”科学技术研究项目(吉教科合字2014第63号) |
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| 摘 要： | 对2014年沈阳地区患有呼吸系统疾病的育肥牛场进行病原分离,得到7株分离菌,对其进行培养形态及染色观察、病理组织切片观察、动物致病性观察及16S序列分析,初步鉴定为牛多杀性巴氏杆菌(Pm)。利用Pm种特异性引物及荚膜血清特异性引物进行PCR扩增,均得到目的基因条带。对分离菌进行药敏试验的结果显示,5株分离菌株已对阿米卡星、新诺明产生较强耐药性;7株分离菌均对氟苯尼考、恩诺沙星、四环素及氨苄西林敏感。由此确定,分离菌株均为牛荚膜A型巴氏杆菌,且已具有一定耐药性。
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| 关 键 词： | 牛多杀性Pm 荚膜血清A型 分离鉴定 耐药性 病理组织学观察 |
| 收稿时间： | 2015-05-11 |
| 修稿时间： | 2015-07-11 |
Isolation,identification and resistance analysis of capsular serotype A pasteurella multocida and histopathological observation |
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| Affiliation: | 1. College of Animal Science and Technology,Jilin Agricultural University, |
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| Abstract: | Isolate and identify the pathogenic from the beef cattle farms which suffered from bovine respiratory disease in Shenyang area in 2014 according to the histopathological, morphology observation and molecular biology method. Further research by the mouse pathogenicity and drug sensitive test. The results indicated that all the lung paraffin section confirmed the lesions were infiltrated by a largenumber of red and granulocyte cells, a particle of fibroblast cells. And all the isolates were identified as capsular serotype A P.multoceda. Drug sensitive test |
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| Keywords: | Pasteurella multocida, capsular serotype A, isolation and identification, resistance, histopathological observation |
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