A Comparison of Genetic Segregation in Traditional and Microspore-Derived Populations of Brassica juncea L. Czern and Coss. |
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Authors: | M. R. Thiagarajah G. R. Stringam |
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Affiliation: | Department of Plant Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada |
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Abstract: | An isolated microspore culture procedure was used to produce doubled haploid lines of Brassica juncea from F1 plants of reciprocal crosses between the cultivar‘RLM514’and a canola quality breeding line. The inheritance of two qualitative markers, seed color and leaf hairiness, was compared using traditional and microspore-derived populations from these crosses. Chi-square tests indicated that each trait is controlled by different sets of duplicate pairs of genes. Brown seeds or hairy leaves can result from the presence of either of two dominant alleles, whereas yellow seed or glabrous leaves are produced when alleles at both loci are recessive. The segregation of genes controlling seed color and leaf hairs in doubled haploid progeny did not differ significantly from that expected under random assortment, indicating that doubled haploids can be used in this species for genetic studies, and probably cultivar development as well. |
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Keywords: | Brassica juncea seed color leaf hairiness microspore culture inheritance doubled haploids |
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