| 新疆稻麦低分子量谷蛋白亚基基因序列分析 |
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| 引用本文： | 江千涛,魏育明,颜泽洪,郑有良. 新疆稻麦低分子量谷蛋白亚基基因序列分析[J]. 四川农业大学学报, 2005, 23(4): 394-397,410 |
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| 作者姓名： | 江千涛 魏育明 颜泽洪 郑有良 |
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| 作者单位： | 四川农业大学小麦研究所,四川,都江堰,611830;四川农业大学教育部作物基因资源与遗传改良重点实验室,四川,雅安,625014 |
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| 基金项目： | 国家自然科学基金项目（30300219）;教育部创新团队发展计划（IRT0453）. |
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| 摘 要： | 根据普通小麦低分子量谷蛋白亚基基因保守区序列,设计了一对引物(P1和P2),采用PCR法对新疆稻麦(Triticum petropavlovskyiUdacz.et Migusch)的基因组DNA进行扩增,获得1条约900 bp的片段,纯化后克隆到载体pMD18-T后,对筛选阳性克隆测序,获得1个基因LMWXJ-1(Genbank登录号:AY695380)。序列分析的结果表明LMWXJ-1具有典型的低分子量谷蛋白亚基基因的基本结构。推导的氨基酸序列比较结果表明,LMWXJ-1与Glu-A3和Glu-D3位点的低分子量谷蛋白基因具有较高的相似性(最高相似性分别为82%和80%),而与Glu-B3位点的差异较大(最高相似性仅有68%)。
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| 关 键 词： | 新疆稻麦 低分子量谷蛋白亚基基因 序列分析 |
| 文章编号： | 1000-2650（2005）04-0394-04 |
| 收稿时间： | 2005-08-16 |
| 修稿时间： | 2005-08-16 |
Sequence Analysis of Low-molecular-weight Glutenin Subunit Gene from Xinjiang Rice Wheat (Triticum petropavlovskyi Udacz.et Migusch) |
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| JIANG Qian-tao,WEI Yu-ming,YAN Ze-hong,ZHENG You-liang. Sequence Analysis of Low-molecular-weight Glutenin Subunit Gene from Xinjiang Rice Wheat (Triticum petropavlovskyi Udacz.et Migusch)[J]. Journal of Sichuan Agricultural University, 2005, 23(4): 394-397,410 |
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| Authors: | JIANG Qian-tao WEI Yu-ming YAN Ze-hong ZHENG You-liang |
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| Affiliation: | 1.Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan 611830, Sichuan, China; 2. Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Siehuan Agricultural University, Yaan 625014, Siehuan, China |
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| Abstract: | In genomic PCR amplification by using primers P1 and P2,one DNA band of approximately 900?bp is amplified for Xinjiang rice wheat accession Daomai 2.After the fragment is recovered and cloned into the pMD18-T vector,one positive clone LMWXJ-1(Genbank accession number: AY695380) with proper insert size is screened and sequenced.LMWXJ-1 has a typical glutenin structure similar to those of published LMW-GS genes.By the deduced amino acid sequence comparison,it is found that LMWXJ-1 has higher similarity to the genes at Glu-A3 and Glu-D3 locus (with the highest similarity 82% and 80% respectively),while it has a lower similarity to the genes at Glu-B3 locus(68%). |
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| Keywords: | Xinjiang rice wheat LMW- GS gene sequence analysis |
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