6月龄和成年牦牛卵巢及卵泡发育状况研究

为了研究6月龄牦牛和成年牦牛卵巢及表面卵泡发育状况,试验比较了6月龄和成年牦牛卵巢长度、宽度、厚度、重量、卵泡数量以及卵母细胞体外成熟培养效果。结果表明:成年牦牛卵巢长度(2.29±0.43)cm、宽度(1.91±1.31)cm和厚度(1.60±1.90)cm均显著大于6月龄牦牛(1.65±0.30)cm、(1.14±0.25)cm、(0.79±0.26)cm](P<0.05),成年牦牛卵巢体积(6.92±7.00)cm3和重量(3.19±1.58)g极显著大于6月龄牦牛体积(1.63±0.93)cm3和重量(0.87±0.44)g(P<0.01)。6月龄牦牛Ⅰ级卵泡数(14.47±8.74)枚和平均总卵泡数(15.17±8.87)枚极显著高于成年牦牛Ⅰ级卵泡数(7.97±3.72)枚和平均总卵泡数(8.98±3.87)枚(P<0.01),Ⅱ级卵泡数差异不显著(P>0.05),成年牦牛平均每头含(0.02±0.15)枚Ⅲ级卵泡,而6月龄牦牛无Ⅲ级卵泡。成年牦牛有黄体卵巢重量显著大于无黄体卵巢重量(P<0.05),有黄体卵巢含(0.06±0.24)枚Ⅲ级卵泡,而无黄体卵巢不含Ⅲ级卵泡。6月龄和成年牦牛A、B级卵母细胞体外培养成熟率分别为(81.39±3.53)%、(80.44±4.50)%,差异不显著(P>0.05),而6月龄牦牛卵巢的平均卵母细胞数和平均A、B级卵母细胞数均显著高于成年牦牛(P<0.05)。

Research on the ovarian and follicular development in six months of age and adult yaks

To investigate the developmental condition of the ovary and ovarian follicles in six months of age and adult yaks,the length,width and thickness of the ovaries and their follicle numbers were compared,the effects of in vitro maturation of yak oocytes were also compared.The results showed that the ovarian length,width and thickness in adult yaks were significantly greater than that of in six months of the age in yaks(P<0.05),the volume and weight in adult yaks were extremely significant higher than that of in six months of age in yaks(P<0.01).ClassⅠ follicular number and average total numbers of follicles in six months of age in yaks were extremely significant higher than that of in adult yaks(P<0.01),but class Ⅱ follicular number were not significantly different.Each of adult yaks contained(0.02±0.15) class Ⅲ follicle,but 6 months of age in yaks had no class Ⅲ follicles.The ovarian weight of adult yak with corpus luteum was significant higher than that of without corpus luteum.At the same time,the ovaries with corpus luteum contained(0.06±0.24) class Ⅲ follicle.The rates of in vitro maturation for class A and B oocytes were(81.39±3.53)% and(80.44±4.50)% in six months of age and adult yaks(P>0.05),while the average oocytes number and average class A and B oocytes number of 6 months of age in yaks was significantly higher than that of adult yaks(P<0.05).