| 粉菠萝体细胞植株再生体系的研究 |
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| 引用本文： | 许早时,卜基保. 粉菠萝体细胞植株再生体系的研究[J]. 安徽农业大学学报, 2012, 39(1): 116-119 |
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| 作者姓名： | 许早时 卜基保 |
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| 作者单位： | 安徽省林业高科开发中心,合肥,230001;安徽省林业高科开发中心,合肥,230001 |
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| 基金项目： | 安徽省林业生态提升关键技术研究和安徽省科技攻关项目(10010302001)共同资助 |
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| 摘 要： | 以粉菠萝冠芽、幼叶为外植体材料,采用外加不同梯度浓度激素的完全培养基(MS)进行组织培养研究。结果表明,MS+BA1.0 mg.L-1+IBA0.1 mg.L-1为最佳丛生芽养基;MS+BA0.2 mg.L-1+NAA 0.1 mg.L-1为最佳芽增殖培养基;1/2 MS+NAA0.5 mg.L-1为最佳生根培养基。通过对再生植株的分子标记研究表明,基因组没有发生变化,表明通过粉菠萝体细胞植株再生苗同粉菠萝原生苗没有明显的差别。
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| 关 键 词： | 组织培养 体细胞 粉菠萝 |
| 收稿时间： | 2011-08-05 |
Study on tissues culture system with somatic cell in Aechmea fasciatacv |
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| XU Zao-shi and BO Ji-bao. Study on tissues culture system with somatic cell in Aechmea fasciatacv[J]. Journal of Anhui Agricultural University, 2012, 39(1): 116-119 |
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| Authors: | XU Zao-shi and BO Ji-bao |
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| Affiliation: | (Institution of Anhui Forestry High-tech Development Center,Hefei 230001) |
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| Abstract: | The shoots and young leaves of Aechmea fasciatacv were used as cell culture material with MS medium plus different concentration hormones.The results show that MS+BA1.0 mg?L-1 + IBA 0.1 mg?L-1 is the best medium for shoots induction;MS+BA 0.2 mg?L-1 +NAA 0.1 mg?L-1 is the best medium for shoot propaga-tion,whereas 1/2MS+NAA 0.5 mg?L-1 is suitable medium for roots induction.PCR amplification shows that ge-nomic DNA of regenerated plants is not obviously different from original Aechmea fasciatacv. |
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| Keywords: | tissue culture somatic cell Aechmea fasciatacv |
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