| 根癌农杆菌介导茶树转基因体系的建立 |
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| 引用本文： | 项威,史成颖,贺志荣,徐燕. 根癌农杆菌介导茶树转基因体系的建立[J]. 安徽农业大学学报, 2012, 39(5): 721-724 |
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| 作者姓名： | 项威 史成颖 贺志荣 徐燕 |
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| 作者单位： | 安徽农业大学教育部和农业部茶叶生物化学与生物技术重点实验室,合肥,230036;安徽农业大学教育部和农业部茶叶生物化学与生物技术重点实验室,合肥,230036;安徽农业大学教育部和农业部茶叶生物化学与生物技术重点实验室,合肥,230036;安徽农业大学教育部和农业部茶叶生物化学与生物技术重点实验室,合肥,230036 |
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| 基金项目： | 国家自然科学基金项目(31070614);安徽农业大学人才引进基金共同资助 |
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| 摘 要： | 利用茶树[Camellia sinensis(L.)O.Kuntze]品种"农抗早"的叶片进行培养,诱导形成愈伤组织。选择生长旺盛、致密的愈伤组织块与根癌农杆菌EHA105进行共培养,通过潮霉素(Hygromycin)抗性筛选、GUS染色及PCR的验证,获得部分转基因愈伤组织。在添加100μmol·L-1的乙酰丁香酮(AS)处理后,转化率约为10%。同时试验结果证明,AS可以提高农杆菌的转化效率,增加渗透压却不能提高转化率。
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| 关 键 词： | 茶树 转基因 组织培养 愈伤组织 根癌农杆菌 |
| 收稿时间： | 2012-05-02 |
Establishment of an agrobacterium-mediated transformation system for tea plant (Camellia sinensis) |
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| XIANG Wei,SHI Cheng-ying,HE Zhi-rong and XU Yan. Establishment of an agrobacterium-mediated transformation system for tea plant (Camellia sinensis)[J]. Journal of Anhui Agricultural University, 2012, 39(5): 721-724 |
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| Authors: | XIANG Wei SHI Cheng-ying HE Zhi-rong XU Yan |
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| Affiliation: | (Key Laboratory of Tea Biochemistry and Biotechnology,Ministry of Education and Agriculture,Anhui Agricultural University,Hefei 230036) |
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| Abstract: | Calli were induced from leaves of tea cultivar ’Nongkangzao’,and those growing vigorously with compact tissue were chosen for agrobacterium-mediated transformation by EHA105.The transgenic callus was screened using hygromycin.The resistant callus was confirmed by RT-PCR and GUS dyeing.The results showed that transgenic callus was obtained using this method,and the conversion rate was about 10% after adding 100 μmol·L-1 acetosyringone(AS).The results also showed that the efficiency of agrobacterium-mediated transforma-tion could be effectively improved by adding AS,but the increase in osmotic pressure had no effect. |
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| Keywords: | Camellia sinensis transgenosis cell tissue culture calli Agrobacterium |
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