| 茶树花粉特异蛋白基因CsPSP的反义载体构建 |
| |
| 引用本文： | 龚莹,余梅,江昌俊,吴凡,吉虎,徐凯,臧洁. 茶树花粉特异蛋白基因CsPSP的反义载体构建[J]. 安徽农业大学学报, 2012, 39(3): 397-400 |
| |
| 作者姓名： | 龚莹 余梅 江昌俊 吴凡 吉虎 徐凯 臧洁 |
| |
| 作者单位： | 安徽农业大学教育部和农业部茶叶生物化学与生物技术重点实验室;安徽农业大学生命科学学院 |
| |
| 基金项目： | 安徽高校省级自然科学研究重点项目计划(KJ2010A119)资助 |
| |
| 摘 要： | 以茶树龙井43品种的后期花蕾为试验材料,提取总RNA,然后进行RT-PCR得到cDNA第1链。同时,根据GenBank上登录的茶树花粉特异蛋白基因CsPSP(DQ887753)的序列,设计1对特异引物进行PCR反应。将扩增后的基因片段插入pBI121表达载体中,并测序。结果表明,插入pBI121载体的片段长度为318 bp,与已知序列进行比对后一致性达到99.02%,说明载体构建成功。
|
| 关 键 词： | 茶树 CsPSP 反义载体 |
Antisense vector construction of CsPSP pollen specific protein gene from tea plant (Camellia sinensis L.) |
| |
| GONG Ying,YU Mei,JIANG Chang-jun,WU Fan,JI Hu,XU Kai and ZANG Jie. Antisense vector construction of CsPSP pollen specific protein gene from tea plant (Camellia sinensis L.)[J]. Journal of Anhui Agricultural University, 2012, 39(3): 397-400 |
| |
| Authors: | GONG Ying YU Mei JIANG Chang-jun WU Fan JI Hu XU Kai ZANG Jie |
| |
| Affiliation: | 1.Key Laboratory of Tea Biochemistry and Biotechnology,Ministry of Education and Agriculture, Anhui Agricultural University,Hefei 230036; 2.School of Life Sciences,Anhui Agricultural University,Hefei 230036) |
| |
| Abstract: | In order to study the expression of the pollen-specific gene in tea plant,a pair of specific primers with double enzyme sites was designed to clone a cDNA fragment of CsPSP gene from Longjing43 by the method of RT-PCR,and then the CsPSP fragment was ligated with pBI121 vector and sequenced.The result indicates that the sequence has 99.02% similarity to CsPSP gene in GenBank. |
| |
| Keywords: | Camellia sinensis. CsPSP antisense vector |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《安徽农业大学学报》浏览原始摘要信息 |
|
点击此处可从《安徽农业大学学报》下载全文 |
|
