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玉米成熟胚和茎尖愈伤诱导及其植株再生能力比较
引用本文:陈莉,窦秉德,白光宏,曹俊梅,朱大伟,张永霞,何晔,孙瑞,侯北伟. 玉米成熟胚和茎尖愈伤诱导及其植株再生能力比较[J]. 新疆农业大学学报, 2006, 29(3): 46-49
作者姓名:陈莉  窦秉德  白光宏  曹俊梅  朱大伟  张永霞  何晔  孙瑞  侯北伟
作者单位:1. 淮阴师范学院,生物系,淮安,223300;新疆农业大学,农学院,乌鲁木齐,830052
2. 新疆农业大学,农学院,乌鲁木齐,830052
3. 淮阴师范学院,生物系,淮安,223300
基金项目:江苏省高校高新技术产业发展项目
摘    要:以3种不同基因型的成熟胚和茎尖两种不同外植体为材料,对它们的愈伤组织诱导,继代培养和分化进行了研究。结果表明:玉米的成熟胚和茎尖愈伤诱导率相关,但两种外植体诱导出的愈伤组织状态明显不同,茎尖诱导出的愈伤组织状态普遍比成熟胚的好;两种外植体的愈伤分化率差异明显,成熟胚愈伤组织最高分化率为2.13%,茎尖愈伤组织最高分化率可达21.2%。

关 键 词:玉米  成熟胚  茎尖  愈伤  再生
文章编号:1007-8614(2006)03-0046-04
修稿时间:2006-05-20

Comparison between Maize Mature Embryo and Shoot Tip Callus Induced and Plant Regeneration
CHEN Li,DOU Bing-de,BAI Guang-hong,CAO Jun-mei,ZHU Da-wei,ZHANG Yong-xia,HE Ye,SUN Rui,HOU Bei-wei. Comparison between Maize Mature Embryo and Shoot Tip Callus Induced and Plant Regeneration[J]. Journal of Xinjiang Agricultural University, 2006, 29(3): 46-49
Authors:CHEN Li  DOU Bing-de  BAI Guang-hong  CAO Jun-mei  ZHU Da-wei  ZHANG Yong-xia  HE Ye  SUN Rui  HOU Bei-wei
Abstract:This paper studies the initiation,maintenance and differentiation of callus from mature embryo and shoot tip of three genotypes in corn.The results showed that maize mature embryo and the callus induced frequency were correlated between mature embryo and shoot tip,but the callus quality was obviously different.Callus obtained from shoot tip were generally better than that of mature embryo.The differentiation frequency had great difference between these two kinds of callus, only 2.13% differentiation frequency could be obtained on mature embryo callus,and the differentiation frequency on shoot tip callus could be up to(21.2%).
Keywords:maize  mature embryo  shoot tip  callus  regeneration
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