番木瓜酸性转化酶基因家族预测及生物信息学初步分析

酸性转化酶参与了番木瓜果实中蔗糖的韧皮部卸载,调控果实中糖类的积累。本文利用番木瓜基因组数据库信息,发现了番木瓜基因组中含有6个酸性转化酶基因,分别为细胞壁转化酶基因（CpCWINV1-3）和液泡转化酶基因（CpVINV1-3）。这些基因由3-7个外显子组成,其中CpCWINV1、CpVINV1和CpVINV2的N端均具有一个跨膜区域。蛋白质3D结构预测分析表明,CpCWINV1、CpVINV1和CpVINV2具有典型的N端β-螺旋桨模块（β-propeller module）和C端β-三明治模块（β-sandwich module）,而CpCWINV3和CpVINV3的桨叶I上,均没有保守区NDPNG/A;CpCWINV2和CpVINV3均缺失了β-三明治模块。推测番木瓜细胞壁转化酶CpCWINV1、液泡转化酶CpVINV1和CpVINV2能正确履行催化蔗糖分解的功能。本研究有助于我们进一步揭示酸性转化酶在番木瓜果实发育及糖类积累过程中的作用。

Gene Family Prediction and Preliminary Analysis of Bioinformatics of Acid Invertase in Carica papaya

Acid invertases involved sucrose phloem unloading and regulate sugars accumulation in papaya fruit. Based on papaya genome database, six acid invertases were found in papaya, cell wall invertase（CpCWINV1-3） and vacuolar invertase member（CpVINV1-3）. The exons numbers of these genes were 3 to 7, and there was an N-terminal transmembrane region in the CpCWINV1, CpVINV1 and CpVINV2. 3D protein structure prediction analysis showed that CpCWINV1, CpVINV1 and CpVINV2 have a typical β-propeller module in N-terminal and β-sandwich module in C-terminal, while CpCWINV3 and CpVINV3, did not have conserved region NDPNG/A in blades I; CpCWINV2 and CpVINV3 are missing β-sandwich module. We speculate that papaya cell wall invertase CpCWINV1, vacuolar invertase CpVINV1 and CpVINV2 were correctly fulfilling the function of the decomposition of sucrose. This study will help us to further reveal the role of acid invertase in papaya fruit development and sugar accumulation.