尖孢镰刀菌（Fusarium oxysporum）乙醛脱氢酶基因的特征分析

尖孢镰刀菌是木质纤维素生物转化乙醇极具潜力微生物菌种。对尖孢镰刀菌基因组中乙醛脱氢酶的研究有助于对其乙醇代谢途径和机理掌握。通过对尖孢镰刀菌基因组数据库的搜索,发现了23条假定乙醛脱氢酶,它们属于醛脱氢酶超家族,并根据其蛋白分子质量大小进行了系统命名。研究分析了这些蛋白的等电点、催化位点、磷酸化位点、亚细胞定位,进行了多序列比对和系统发育树构建,并获得了一条乙醛脱氢酶基因的核苷酸序列,这为尖孢镰刀菌乙醛脱氢酶基因的功能验证及乙醇代谢机理研究奠定了基础。

The Characteristic Analysis of Aldehyde Dehydrogenase Genes from Fusarium oxysporum

Fusarium oxysporum is a potential microbial strain, which can convert the lignocellulose materials into bioethanol. Investigation to acetaldehyde dehydrogenases from F. oxysporum genome could facilitate to understand the pathway and mechanism of its ethanol metabolism. In this paper, genome-wide analysis of acetaldehyde dehydrogenase genes in F. oxysporum genome database revealed that the presence of 23 putative acetaldehyde dehydrogenase, all of them belong to a aldehyde dehydrogenase superfamily. These aldh from F. oxysporum were named according to the molecular weight of protein. Isoelectric point, catalytic site, phosphorylation sites, subcellular localization of these proteins were analyzed. To investigate the homology and phylogenetic relationship of these acetaldehyde dehydrogenase, the multiple sequence alignment and phylogenetic trees were established. Furthermore, a nucleotide sequence of acetaldehyde dehydrogenase gene was cloned. These works provide the foundation for the functional verification of acetaldehyde dehydrogenase genes from F. oxysporum and for metabolic mechanism of ethanol about F. oxysporum.