| 新疆野扁桃自交不亲和花粉SFB基因的克隆及生物信息学分析 |
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| 引用本文： | 曾斌,刘楠楠,夏江宏,刘梦雯,王建友,王波. 新疆野扁桃自交不亲和花粉SFB基因的克隆及生物信息学分析[J]. 中国农学通报, 2017, 33(25): 22-30 |
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| 作者姓名： | 曾斌 刘楠楠 夏江宏 刘梦雯 王建友 王波 |
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| 作者单位： | 新疆农业大学林学与园艺学院/新疆农业大学果树学新疆特色果树研究中心,新疆应用职业技术学院园林园艺系,新疆农业大学林学与园艺学院/新疆农业大学果树学新疆特色果树研究中心,新疆农业大学林学与园艺学院/新疆农业大学果树学新疆特色果树研究中心,中国林业科学院新疆分院,新疆农业大学林学与园艺学院/新疆农业大学果树学新疆特色果树研究中心 |
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| 基金项目： | 国家自然科学基金“基于S-核酸酶的新疆野生扁桃自交不亲和性泛素化特性的分子机制研究(31660557)”，“新疆野生扁桃自交不亲和性 的分子机制研究”(31260465)；新疆维吾尔自治区果树学重点学科项目“新疆特色果树种质资源开发利用与优良品种选育推广”(201007)。 |
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| 摘 要： | 旨在克隆获得新疆野扁桃自交不亲和花粉SFB新基因,以新疆野扁桃花药为试材,应用分子同源克隆及RT-PCR等技术,并进一步采用在线软件分析SFB基因的蛋白质理化性质,实验成功克隆到了PtSFB5与PtSFB6 2个新SFB基因的cDNA全长,2个基因的cDNA全长分别为1124 bp和1072 bp,分别编码了374和348个氨基酸,2个基因都具有F-Box基因结构,属于F-Box基因家族,预测的相对分子量分别为30787.26和44037.74,等电点为5.93和5.43,均属于水溶性不稳定蛋白。
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| 关 键 词： | 新疆野扁桃 自交不亲和 SFB基因 克隆 生物信息学 |
| 收稿时间： | 2016-10-24 |
| 修稿时间： | 2016-12-13 |
Molecular Cloning and Bioinformatics Analysis of SFB Genes Controlling Self-incompatibility in Xinjiang Wild Almond (Prunus tenella Batsch.) |
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| Abstract: | The study aims to clone full-length sequence of the new pollen SFB gene of self-incompatibility in Prunus tenella. Xinjiang wild almond was used as the material, and molecular homologous cloning and RT-PCR were conducted in this study. The online software was used to analyze the protein physicochemical properties of SFB. The full-length gene sequences of PtSFB5 and PtSFB6 were successfully cloned. The full-length sequence of the two SFB genes was 1124 bp and 1072 bp, respectively, and encoded 374 aa and 348 aa, respectively. Both of the two genes had F- Box structure, belonged to the F- Box gene family. The predicted relative molecular mass was 30787.26 and 44037.74, and the isoelectric point was 5.93 and 5.43, respectively. PtSFB5 and PtSFB6 protein belonged to the unstable water-soluble protein. |
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| Keywords: | Prunus tenella Batsch. self-incompatibility SFB gene cloning bioinformatics |
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