血水草基因组DNA提取方法的优化

[目的]优化血水草（Eomecon chionantha Hance）基因组DNA提取方法。[方法]采用常规CTAB法和改良CTAB法提取血水草基因组DNA。[结果]常规CTAB法提取的DNA含杂质多、难溶解、易降解;而改良的CTAB方法适合血水草基因组DNA的提取,采用鲜嫩叶样品和-80℃硅胶干燥嫩叶样品均可能获得完整性较好、纯度较高的基因组DNA。[结论]为血水草遗传多样性研究奠定了基础。

Optimization of Extraction Methods for Genomic DNA on Eomecon chionantha Hance

[Objective] The research aimed to optimize the extraction methods for genomic DNA on Eomecon chionantha Hance.[Method] The methods of CTAB and modified CTAB were chose to extract the genomic DNA on Eomecon chionantha Hance leaves.[Result] The results showed that the genomic DNA extracted by CTAB method contained many impurity,were difficult to dissolve and easily degradable.The modified CTAB method was suitable to extract the genomic DNA on Eomecon chionantha Hance.The concentration and extraction rate of DNA from both fresh leaves and dry leaves preserved with silica gel at -80 ℃ were higher.[Conclusion] The study can provide the basis for studying the genetic diversity of Eomecon chionantha Hance.