芜菁离体培养再生体系建立

【目的】研究不同激素对芜菁再生不定芽,不定根的影响;并建立芜菁再生体系。【方法】设置2种浓度NAA激素0.1 mg/L,0.5 mg/L;和4种浓度6-BA激素0.25、0.5、0.75、1.0 mg/L处理2种新疆芜菁、1种温州盘菜的子叶、下胚轴。观察记录子叶、下胚轴的不定芽再生频率和再生根频率。【结果】三种芜菁下胚轴产生不定芽的频率为:柯坪芜菁(75.32%)>温州盘菜(73.73)%>喀什芜菁(72.55%)。NAA为0.5 mg/L时下胚轴的生根率要高于NAA为0.1 mg/L时的生根率。【结论】三种芜菁的下胚轴诱导不定芽的效果要优于子叶的诱导效果;柯坪芜菁与喀什芜菁不定芽诱导最适激素浓度为6-BA 0.75 mg/L、NAA 0.1 mg/L;温州盘菜最适激素浓度为6-BA 0.5 mg/L、NAA 0.1 mg/L。

Establishment of Isolated Culture Regeneration System of Brassica rapa L.

【Objective】 To explore the influence of different hormones on the adventitious buds and roots of turnips (Brassica rapa L.) and establish turnips regeneration system.【Method】 Two concentrations of NAA 0.1 mg / L and 0.5 mg / L and 4 concentrations of 6 - BA hormone 0.25 , 0.5 , 0.75 , 1.0 mg / L were set up to treat the cotyledons and hypocotyls of two kinds of Xinjiang turnips, one kind of Wenzhou for observing the frequency of adventitious bud regeneration and regenerated roots. 【Result】The regeneration rate of three hypocotyls was: Keping (75.32%) > Wenzhou (73.73%) > Kashi (72.55%). When NAA was at 0.5 mg/L, the hypocotyls rooting rate was higher than that when NAA was at 0.1 mg/L.【Conclusion】 The effect of Hypocotyl inducing adventitious bud of three kinds of turnip was better than that cotyledons. The optimal hormone concentrations of adventitious bud induction turnips in Keping and Kashi are 6-BA 0.75 mg/L and NAA 0.1 mg/L. and the optimal hormone concentration of Wenzhou dish is 6-BA 0.5 mg/L and NAA 0.1 mg / L.