不同产地库尔勒香梨萼端黑斑病 病原菌的分离鉴定

【目的】 明确阿克苏地区和巴音郭楞蒙古自治州地区库尔勒香梨萼端黑斑病是否由同一种致病菌引起的,以两个产地库尔勒香梨萼端黑斑病病果为研究对象,研究两个产地萼端黑斑病的致病种类,为该病害的防控奠定前期基础。【方法】 采用划线培养法培养菌丝进行形态学鉴定,通过基因组DNA提取,18S菌保守序列PCR扩增和3 730测序进行萼端黑斑病病原菌的生物学鉴定。【结果】 确定是单一菌还是混合菌,并对优势或主导病原菌菌群进行形态学及分子生物学鉴定,为该病害的防控提供基础理论依据。【结论】 两个产地库尔勒香梨萼端黑斑病的致病菌为单一菌,并且都是链格孢属交链孢菌(Alternaria alternate(Fr.)Keissl)。

The Separation and Identification of the Pathogenic Bacteria of Korla Pear Calyx End Black Spot Disease in Different Growing Areas

【Objective】 To explore whether the black spot of Korla pear in Aksu and Bazhou were caused by the same pathogenic bacteria,which might lay a foundation for the prevention and control of this disease in the future. The article used the calyx end black spot Korla pear in the Aksu Area area of aksu and bazhou Bazhou Area as testing material to separate and purify pathogenic bacteria from the diseased fruit to study the pathogenic types of black spot of calyx.【Method】 The mycelium was cultured with the method of underlined culture for morphological identification and biological identification of calyx end black spot disease was carried out through genomic DNA extraction, PCR amplification of 18S conserved sequence and 3,730 sequencing. 【Result】 Which was determined whether was a single fungus or mixed bacteria, and to makeidentified as morphological and molecular biological identification of the dominant or a dominant pathogenic microflora in terms of morphology and molecular biology. This result provided the theoretical basis for the prevention and control of the disease. 【Conclusion】 The results showed that the pathogenic bacteria of Korla pear calyx end black spot disease in the two growth areas of were monobacteria and were consistent with Alternaria alternate(Fr.)Keissl.