哈萨克羊MSTN基因的DNA甲基化分析

【目的】研究肌肉和脂肪组织中MSTN基因(生长抑素)的DNA甲基化水平,为改良哈萨克羊品种的选育提供遗传科学依据。【方法】基于亚硫酸氢盐的方法,测序哈萨克绵羊MSTN基因在CpG二核苷酸中的DNA甲基化水平。【结论】6月龄哈萨克羊肌肉MSTN基因DNA甲基化低,脂肪组织DNA甲基化程度最高,股二头肌,股三头肌,半膜肌,半腱肌,背最长肌和脂肪组织的MSTN基因的DNA甲基化概率分别为34.7％,22.6％,23.3％,28％,42.6％和76.7％。【结论】哈萨克羊各组织MSTN基因DNA的甲基化概率没有显著差异。脂肪组织MSTN基因DNA甲基化的最高,肌肉组织MSTN基因的DNA甲基化的概率较低。脂肪的甲基化概率是背最长肌,股二头肌,股三头肌,半腱肌,半膜肌的甲基化概率分别为:1.8倍,2.2倍,3.39倍,2.74倍,3.29倍。其次甲基化从高到低的依次为:脂肪组织>背最长肌>股二头肌>半腱肌>半膜肌>股三头肌。

Analysis of DNA Methylation of MSTN Gene in Kazak Sheep

【Objective】 To study the DNA methylation level of MSTN (spermatostatin) gene in muscle and adipose tissue, and to provide genetic data for improving the breeding of Kazakh sheep.【Method】The bisulfite treatment sequencing method using whole genome methylation analysis was used to determine the DNA methylation level of MSTN gene of Kazak sheep in CpG dinucleotide acid.【Result】The MSTN gene DNA methylation was low in 6-month-old Kazak sheep muscle, and the highest DNA methylation degree in adipose tissue. The DNA methylation probability of MSTN gene in biceps femoris muscle, triceps femoris muscle, semimembranous muscle, semitendinosus muscle, longissimus dorsi muscle and adipose tissue were as follows: 34.7%, 22.6%, 23.3%, 28%, 42.6%, and 76.7%, respectively.【Conclusion】There is no significant difference in methylation probability of MSTN gene DNA between Kazakh sheep tissues. The methylation probability of fat is the highest, and the methylation probability of muscle tissue is low. The methylation probability of fat is the longest in the back, the methylation probabilities of the femoral second muscle, the trigeminal muscle, the semitendinosus, and the semimembrane muscle are 1.8, 2.2, 3.38, 2.72, and 3.27 times, respectively, followed by adipose tissue ≥ longissimusdorsi muscle ≥ femoral muscle ≥ semitendinosus ≥ semimembranous muscle ≥ trigeminal muscle.