移植脐带间充质干细胞对荷斯坦公犊日增重及血清生长因子水平的影响

本试验旨在研究移植脐带间充质干细胞对荷斯坦公犊日增重及血清生长因子水平的影响.选取1月龄体重相近的荷斯坦公犊28头,随机分为对照组和试验组,采集健康的荷斯坦新生胎牛的脐带组织,通过胰酶消化筛选法体外分离、纯化及培养牛脐带间充质干细胞(UC-MSCs),试验组按公犊体重(3×10⁵个/kg干细胞)稀释至8 mL颈静脉注射至体内,对照组注射等量生理盐水.试验期154 d.结果表明,注射UC-MSCs后,试验组公犊平均体重在4、5、6月龄时显著高于对照组(P< 0.05);试验组公犊平均日增重在3、4月龄时显著高于对照组(P< 0.05),而在6月龄时极显著高于对照组(P< 0.01).试验组血清中类胰岛素样生长因子(IGF-1)水平在4月龄时显著高于对照组(P< 0.05);试验组血清中血管内皮生长因子(VEGF)、转化生长因子(TGF-α)水平在3月龄时显著高于对照组(P< 0.05);试验组血清中VEGF、成纤维细胞生长因子(bFGF)、TGF-α水平在6月龄时显著或极显著高于对照组(P< 0.05;P< 0.01);2、3、4、6月龄时,试验组血清中骨形态发生蛋白(BMP-7)水平显著低于对照组(P< 0.05).综上所述,移植UC-MSCs能显著提高公犊的平均日增重及血清中IGF-1、VEGF、TGF-α、bFGF水平,血清中这些生长因子与公犊的生长发育密切相关,进而影响其生长性能.

Effect on Average Daily Weight Gain and Growth Factor Levels in the Serum of Holstein Bull Calf after Injecting Umbilical Cord Mesenchymal Stem Cells

This study was aimed to explore the effect on average daily weight gain and growth factor levels in the serum of Holstein bull calf after injecting umbilical cord mesenchymal stem cells (UC-MSCs).28 Holstein bull calves were selected with conditions of be born for about one month and similar weight, then they were divided into control and experimental groups.The test collected healthy fresh Holstein cow fetal umbilical cord organization, purificated and cultivated UC-MSCs in vitro by the method of pancreatic enzyme digestion.Calves of experimental group was injected in jugular vein with the successful UC-MSCs which diluted to 8 mL (3×10⁵ cells/kg), the control group was injected with saline.The test lasted for 154 d.The results showed that after injecting UC-MSCs, the average weight of experimental group was significantly higher than that of control group in 4, 5 and 6 months (P< 0.05), the average daily gain of experimental group was significantly higher than that of control group in 3 and 4 months (P< 0.05), while the difference was very significant in 6 months (P< 0.01).Serum insulin-like growth factor (IGF-1) level of experimental group was significantly higher than that of control group in 4 months (P< 0.05).Serum vascular endothelial growth factor (VEGF), transforming growth factor (TGF-α) levels of experimental group were significantly higher than that of control group in 3 months (P< 0.05).Serum VEGF, bFGF, TGF-α levels of experimental group were significantly or very significantly higher than that of control group (P< 0.05;P< 0.01).Serum BMP-7 level of experimental group was significantly lower than that of control group in 2, 3, 4 and 6 months (P< 0.05).In conclusion, UC-MSCs transplantation significantly improved male calf serum IGF-1, VEGF, TGF-α and bFGF levels, these growth factors and male calf serum in the growth and development were closely related, and then influence the growth performance.